Kwon T-R, Yoo K H, Oh C T, Shin D H, Choi E J, Jung S J, Hong H, Choi Y S, Kim B J
Department of Medicine, Graduate School, Chung-Ang University, Seoul, Korea; Department of Dermatology, Chung-Ang University College of Medicine, Seoul, Korea.
Skin Res Technol. 2015 May;21(2):192-200. doi: 10.1111/srt.12176. Epub 2014 Sep 15.
BACKGROUND/AIMS: Cryolipolysis is a noninvasive method for the selective reduction of localized fat tissues. It has demonstrated efficacy in both clinical and preclinical trials; however, despite its popularity, its mechanisms of action and evaluation methods are not yet fully defined. The purpose of this study was to improved methods for cryolipolysis using a porcine model.
The abdomens of female PWG micro-pigs were treated with a cooling device (CRYOLIPO II(™)), and we examined the treatment effects using photography, three-dimensional photography, ultrasound, gross, and microscopic pathology, and serum lipid level analyses in order to determine the mechanism of action, efficacy, and safety of CRYOLIPO II(™).
CRYOLIPO II(™) successfully reduced abdominal fat in our porcine model. Gross and microscopic histological results confirmed the noninvasive cold-induced selective subcutaneous fat destruction, and showed increases in pre-adipocyte differentiation and in the activation of lipid catabolism. In particular, we found that CRYOLIPO II(™) may increase PPARδ (delta) levels in adipose tissue at 30-60 days post-treatment.
Fat reduction by cryolipolysis was successfully achieved in our porcine model. Thus, our findings indicate that CRYOLIPO II(™) may be a promising fat reduction device for body contouring and fat reduction in humans, and that cryolipolysis exerts its effects, at least partly, by targeting the PPARδ signaling pathway. These results show that both investigative and diagnostic potentials capacity.
背景/目的：冷冻溶脂术是一种用于选择性减少局部脂肪组织的非侵入性方法。它在临床和临床前试验中均已证明有效；然而，尽管其很受欢迎，但其作用机制和评估方法尚未完全明确。本研究的目的是利用猪模型改进冷冻溶脂术的方法。
使用冷却装置（CRYOLIPO II(™)）对雌性PWG微型猪的腹部进行治疗，我们通过摄影、三维摄影、超声、大体和显微镜病理学检查以及血清脂质水平分析来研究治疗效果，以确定CRYOLIPO II(™)的作用机制、疗效和安全性。
CRYOLIPO II(™)在我们的猪模型中成功减少了腹部脂肪。大体和显微镜组织学结果证实了非侵入性冷诱导的选择性皮下脂肪破坏，并显示前脂肪细胞分化增加以及脂质分解激活。特别是，我们发现CRYOLIPO II(™)在治疗后30 - 60天可能会增加脂肪组织中PPARδ（δ）的水平。
我们的猪模型成功实现了通过冷冻溶脂术减少脂肪。因此，我们的研究结果表明CRYOLIPO II(™)可能是一种有前景的用于人体塑形和减脂的减脂设备，并且冷冻溶脂术至少部分通过靶向PPARδ信号通路发挥作用。这些结果显示了其研究和诊断潜力。
