Cortés R, Gueye B, Pazos A, Probst A, Palacios J M
Preclinical Research, Sandoz Ltd, Basle, Switzerland.
Neuroscience. 1989;28(2):263-73. doi: 10.1016/0306-4522(89)90178-4.
The distribution of dopamine D1 receptors has been determined in post mortem human brain tissues using in vitro receptor autoradiography, with ([3H]N-methyl) SCH 23390 as ligand. The highest densities of dopamine D1 sites were seen in the nucleus caudatus, putamen, globus pallidus pars medialis and substantia nigra. Intermediate densities were associated with the amygdala, mammillary bodies, cerebral cortex and CA1. The remaining part of the hippocampus as well as the diencephalon, brainstem and cerebellum contained low levels of [3H]SCH 23390 binding sites. The distribution of D1 receptors in the human brain closely resembles that reported for the rat brain. In addition, there was a good correlation between the anatomical localization of D1 sites and the distribution of dopaminergic nerve terminals in the central nervous system. The densities of D1 receptors in the human brain were observed to markedly decrease with age during the first decades of life. However, no further modifications were found beyond the age of 40 years. We did not observe any significant influence of other parameters such as gender and post mortem delay in our samples.
利用体外受体放射自显影技术，以([3H]N-甲基)SCH 23390作为配体，已确定了多巴胺D1受体在人死后脑组织中的分布情况。在尾状核、壳核、苍白球内侧部和黑质中观察到多巴胺D1位点的密度最高。中等密度与杏仁核、乳头体、大脑皮质和CA1相关。海马体的其余部分以及间脑、脑干和小脑含有低水平的[3H]SCH 23390结合位点。人脑中D1受体的分布与大鼠脑的报道非常相似。此外，D1位点的解剖定位与中枢神经系统中多巴胺能神经末梢的分布之间存在良好的相关性。在生命的最初几十年中，观察到人类大脑中D1受体的密度随年龄显著降低。然而，40岁以后未发现进一步的变化。在我们的样本中，未观察到性别和死后延迟等其他参数的任何显著影响。
