Palacios J M, Camps M, Cortés R, Probst A
Preclinical Research, Sandoz Ltd, Basle, Switzerland.
J Neural Transm Suppl. 1988;27:227-35. doi: 10.1007/978-3-7091-8954-2_20.
We have investigated the anatomic localization of dopamine D1 and D2 receptors in the human brain using selective high affinity ligands for both types of dopamine receptors and the technique of receptor autoradiography. Dopamine D1 receptors were labeled in postmortem human brain tissue sections using the antagonist [3H]SCH 23390. Dopamine D2 receptors were labeled in consecutive tissue sections using the agonist [3H]205-502 and the antagonist [3H]spiroperidol. D1 and D2 dopamine receptors presented a heterogeneous distribution in the human brain. The highest concentrations of both D1 and D2 receptors were found in parts of the basal ganglia, particularly the nucleus caudatus and putamen. Lower concentrations were seen in other areas for example, the lateral globus pallidus was enriched in D2 receptors and the medial globus pallidus in D1 receptors. The substantia nigra contained intermediate densities of both D1 and D2, D1 receptors being present in higher concentrations. Dopamine D1 receptors were also localized in areas outside of the basal ganglia, particularly in the neocortex, amygdala and hippocampal formation. Dopamine D2 receptors were also present in areas outside of the basal ganglia, the most significant densities being found in the hippocampal formation. We observed a marked age-dependent decline in the density of D1 receptors during the first decades of life. In contrast, D2 receptor concentrations appeared to be unaltered with age. The distribution and densities of dopamine receptors were examined in 12 cases of Parkinson's disease and compared to a control adult population. No significant differences in density and distribution were seen for either D1 nor D2 receptors.
我们使用针对两种类型多巴胺受体的选择性高亲和力配体及受体放射自显影技术，研究了多巴胺D1和D2受体在人脑内的解剖定位。利用拮抗剂[3H]SCH 23390对死后的人脑组织切片中的多巴胺D1受体进行标记。利用激动剂[3H]205 - 502和拮抗剂[3H]螺哌啶醇对连续的组织切片中的多巴胺D2受体进行标记。D1和D2多巴胺受体在人脑中呈现出异质性分布。在基底神经节的部分区域，尤其是尾状核和壳核，发现了最高浓度的D1和D2受体。在其他区域浓度较低，例如，外侧苍白球富含D2受体，内侧苍白球富含D1受体。黑质含有中等密度的D1和D2受体，D1受体浓度更高。多巴胺D1受体也定位于基底神经节以外的区域，尤其是新皮层、杏仁核和海马结构。多巴胺D2受体也存在于基底神经节以外的区域，在海马结构中发现的密度最为显著。我们观察到在生命的最初几十年中，D1受体密度呈现出明显的年龄依赖性下降。相比之下，D2受体浓度似乎不会随年龄变化。在12例帕金森病患者中检查了多巴胺受体的分布和密度，并与对照成年人群进行比较。D1和D2受体在密度和分布上均未观察到显著差异。
