Zaher Ahmed M, Makboul Makboul A, Moharram Ahmad M, Calderón Angela I
Department of Drug Development and Discovery, Harrison School of Pharmacy, Auburn University, 4306 Walker Building, Auburn, AL 36849, USA; Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt.
Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt.
J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Nov 1;970:18-23. doi: 10.1016/j.jchromb.2014.08.037. Epub 2014 Sep 6.
An endophyte Bartalinia pondoensis Marinc of Citrus aurantum L. var. dulcis was isolated and studied for its secondary metabolites and for their Mycobacterium tuberculosis shikimate kinase (MtSK) inhibitory activities. Using LC-MS metabolite fingerprinting of the constituents of the methanol extract, 19 compounds pertaining to various classes were identified: amino acids, proto-alkaloids, fatty acid amides and oxazole, aniline derivatives and aromatic compounds. We report here for the first time the presence of the [N-(ethyloxy, hydroxymethyl)phenylethylamine] as a new proto-alkaloid and 18 other known compounds are reported for the first time in the genus of Bartalinia. MtSK inhibitory activities of methanol extract and fractions obtained by solid phase extraction (SPE) at a concentration of 50 μg/mL may be attributed to the presence of aniline and oxazole derivatives present in all fractions in varying concentrations.
从甜橙（Citrus aurantum L. var. dulcis）中分离出一种内生真菌巴塔林尼亚蓬多ensis（Bartalinia pondoensis Marinc），并对其次生代谢产物及其对结核分枝杆菌莽草酸激酶（MtSK）的抑制活性进行了研究。通过对甲醇提取物成分进行液相色谱 - 质谱代谢物指纹图谱分析，鉴定出19种属于不同类别的化合物：氨基酸、原生物碱、脂肪酸酰胺和恶唑、苯胺衍生物及芳香族化合物。我们首次在此报道了[N -（乙氧基，羟甲基）苯乙胺]作为一种新的原生物碱的存在，并且首次在巴塔林尼亚属中报道了其他18种已知化合物。甲醇提取物和通过固相萃取（SPE）获得的馏分在浓度为50μg/mL时对MtSK的抑制活性可能归因于所有馏分中不同浓度存在的苯胺和恶唑衍生物。
