Department of Biotechnology, Thapar Institute of Engineering and Technology, Patiala, 147004, Punjab, India.
Vivekananda Institute of Tropical Mycology (VINSTROM), Ramakrishna Mission Vidyapith, Chennai, 600004, Tamil Nadu, India.
Folia Microbiol (Praha). 2022 Dec;67(6):955-964. doi: 10.1007/s12223-022-00991-4. Epub 2022 Jul 29.
Endophytic fungi in plant tissues produce a wide range of secondary metabolites and enzymes, which exhibit a variety of biological activities. In the present study, litter endophytic fungi were isolated from a fire-prone forest and screened for thermostable cellulases. Among nine endophytic fungi tested, two isolates, Bartalinia pondoensis and Phoma sp., showed the maximum cellulase activity. Bartalinia pondoensis was further selected for its cellulase production and characterization. Among the carbon and nitrogen sources tested, maximum cellulase production was observed with maltose and yeast extract, and the eucalyptus leaves and rice bran served as the best natural substrates. The cellulase activity increased with increasing temperature, with maximum activity recorded at 100 °C. The maximum CMCase activity was observed between pH 6.0 and 7.0 and retained 80% of its activity in the pH range of 8-10. Partially purified cellulase of B. pondoensis retained 50% of its activity after 2 h of incubation at 60 °C, 80 °C and 100 °C. These results suggest that litter endophytic fungus B. pondoensis is a potential source for the production of thermostable and alkali-tolerant cellulase.
植物组织中的内生真菌会产生多种次生代谢物和酶，具有多种生物活性。在本研究中，从易发生火灾的森林中分离出凋落物内生真菌，并对其耐热纤维素酶进行了筛选。在所测试的 9 种内生真菌中，有 2 种分离株，即巴塔林亚pondoensis 和 Phoma sp.，表现出最大的纤维素酶活性。进一步选择巴塔林亚pondoensis 进行纤维素酶的生产和特性研究。在测试的碳源和氮源中，麦芽糖和酵母提取物的纤维素酶产量最高，而桉树叶和米糠则作为最佳的天然底物。纤维素酶活性随温度的升高而增加，在 100°C 时达到最大值。CMCase 活性在 pH 值 6.0 到 7.0 之间达到最大值，并在 pH 值为 8 到 10 之间保留 80%的活性。B. pondoensis 的部分纯化纤维素酶在 60°C、80°C 和 100°C 下孵育 2 小时后，保留了 50%的活性。这些结果表明，凋落物内生真菌 B. pondoensis 是生产耐热和耐碱纤维素酶的潜在来源。
