Farha Arakkaveettil Kabeer, Hatha Abdulla Mohamed
Department of Marine Biology Microbiology and Biochemistry, School of Marine Sciences, Cochin University of Science and Technology (CUSAT), Kochi, India.
Mycology. 2019 Jan 31;10(2):109-117. doi: 10.1080/21501203.2019.1572034. eCollection 2019 Jun.
Marine fungi, one of the major decomposers of marine environment, is found to produce potential enzymes and novel biomolecules. The present study explored bioprospecting potentials such as antimicrobial, anticancer and enzymatic activities of marine sediment-derived fungi isolated from continental slope of Eastern Arabian Sea. Morphology and ITS sequencing identified the fungus as sp. ArCSPf. The fungal strain exhibited amylase, gelatinase, phytase, lipase and pectinase activity. The active fraction obtained from the ethyl acetate extract column fractionation (F2) of fungus showed antibacterial activity against both methicillin-resistant (MRSA) and . Minimum inhibitory concentrations of F2 were 125μg/mL for MRSA and 62.5μg/mL for . The active fraction showed a significant anticancer activity ( µg/mL) against MCF-7 breast cancer cells. The secondary metabolite (Z)-Octadec-9-enamide (oleamide, / 282.27 (M + H)] was identified in the LC-MS/MS analysis of active fraction F2 in positive ionisation mode. To the best of our knowledge, this is the first report on exploring the bioprospecting potential of a sediment-derived fungus from continental slope of eastern Arabian Sea for the production of therapeutically active compounds.
海洋真菌是海洋环境中的主要分解者之一，被发现能产生潜在的酶和新型生物分子。本研究探索了从阿拉伯海东部大陆坡分离出的海洋沉积物源真菌的生物勘探潜力，如抗菌、抗癌和酶活性。通过形态学和ITS测序将该真菌鉴定为ArCSPf菌属。该真菌菌株表现出淀粉酶、明胶酶、植酸酶、脂肪酶和果胶酶活性。从真菌的乙酸乙酯提取物柱分馏物（F2）中获得的活性部分对耐甲氧西林金黄色葡萄球菌（MRSA）和[此处原文缺失信息]均表现出抗菌活性。F2对MRSA的最低抑菌浓度为125μg/mL [此处原文缺失信息]为62.5μg/mL。该活性部分对MCF-7乳腺癌细胞表现出显著的抗癌活性（[此处原文缺失μg/mL具体数值]）。在正离子模式下对活性部分F2进行LC-MS/MS分析时，鉴定出次生代谢产物（Z）-十八碳-9-烯酰胺（油酰胺，[此处原文缺失具体分子量信息]/ 282.27（M + H）]。据我们所知，这是关于探索阿拉伯海东部大陆坡沉积物源真菌生产治疗活性化合物的生物勘探潜力的首次报道。
