基于环介导等温扩增法检测不动杆菌临床分离株中碳青霉烯酶携带情况的评估
Evaluation of a loop-mediated isothermal amplification-based methodology to detect carbapenemase carriage in Acinetobacter clinical isolates.
作者信息
Vergara Andrea, Zboromyrska Yuliya, Mosqueda Noraida, Morosini María Isabel, García-Fernández Sergio, Roca Ignasi, Cantón Rafael, Marco Francesc, Vila Jordi
机构信息
Department of Clinical Microbiology, CDB, Hospital Clinic, School of Medicine, University of Barcelona, Barcelona, Spain.
Centre for International Health Research (CRESIB-Hospital Clinic), Barcelona, Spain.
出版信息
Antimicrob Agents Chemother. 2014 Dec;58(12):7538-40. doi: 10.1128/AAC.03870-14. Epub 2014 Sep 15.
Abstract
Carbapenem-resistant Acinetobacter baumannii is a major source of nosocomial infections worldwide and is mainly associated with the acquisition of OXA-type carbapenemases and, to a lesser extent, metallo-β-lactamases (MBLs). In this study, 82 nonepidemiologically related Acinetobacter strains carrying different types of OXA or MBL enzymes were tested using the Eazyplex system, a loop-mediated isothermal amplification (LAMP)-based method to rapidly detect carbapenemase carriage. The presence/absence of carbapenem-hydrolyzing enzymes was correctly determined for all isolates in <30 min.
摘要
耐碳青霉烯鲍曼不动杆菌是全球医院感染的主要来源，主要与获得OXA型碳青霉烯酶有关，在较小程度上与金属β-内酰胺酶（MBLs）有关。在本研究中，使用Eazyplex系统（一种基于环介导等温扩增（LAMP）的方法来快速检测碳青霉烯酶携带情况）对82株携带不同类型OXA或MBL酶且无流行病学关联的不动杆菌菌株进行了检测。在不到30分钟的时间内就正确确定了所有分离株中是否存在碳青霉烯水解酶。
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