Iyalomhe Osigbemhe, Khantwal Chandra M, Kang Di Cody
Geriatrics Division, Department of Medicine, Howard University Hospital, 2041 Georgia Ave NW, Washington, DC, 20060, USA,
J Membr Biol. 2015 Aug;248(4):641-50. doi: 10.1007/s00232-014-9728-y. Epub 2014 Sep 16.
OxlT, the oxalate transporter of Oxalobacter formigenes, is a member of the Major Facilitator Superfamily of transporters (MFS), one of the largest groups of membrane proteins with substantial relevance to solute transport physiology, pharmacology, and possible drug development. MFS proteins transport a wide range of substrates such as organic and inorganic anions, sugars, drugs, and neurotransmitters. This review succinctly summarizes experimental work on a model MFS protein, OxlT, beginning with its identification as an electrogenic oxalate/formate exchanger, its three-dimensional structure, and discussion of biochemical and biophysical data that have shed further light on its structure and function. We also discuss the structure and function of OxlT in relation to notable MFS carriers such as LacY and GlpT.
甲酸草酸杆菌的草酸转运蛋白OxlT是主要转运体超家族（MFS）的成员之一，MFS是最大的膜蛋白家族之一，与溶质转运生理学、药理学及可能的药物开发密切相关。MFS蛋白可转运多种底物，如有机和无机阴离子、糖类、药物及神经递质。本综述简要总结了关于模型MFS蛋白OxlT的实验工作，首先介绍了其作为电生性草酸/甲酸交换体的鉴定、三维结构，以及对其结构和功能有进一步阐明作用的生化和生物物理数据的讨论。我们还讨论了OxlT与著名的MFS载体（如LacY和GlpT）相关的结构和功能。
