用于下一代测序的唾液DNA的收集与提取。
Collection and extraction of saliva DNA for next generation sequencing.
作者信息
Goode Michael R, Cheong Soo Yeon, Li Ning, Ray William C, Bartlett Christopher W
机构信息
Battelle Center for Mathematical Medicine, The Research Institute at Nationwide Children's Hospital.
Battelle Center for Mathematical Medicine, The Research Institute at Nationwide Children's Hospital; The Interdisciplinary Graduate Program in Biophysics, The Ohio State University.
出版信息
J Vis Exp. 2014 Aug 27(90):51697. doi: 10.3791/51697.
Abstract
The preferred source of DNA in human genetics research is blood, or cell lines derived from blood, as these sources yield large quantities of high quality DNA. However, DNA extraction from saliva can yield high quality DNA with little to no degradation/fragmentation that is suitable for a variety of DNA assays without the expense of a phlebotomist and can even be acquired through the mail. However, at present, no saliva DNA collection/extraction protocols for next generation sequencing have been presented in the literature. This protocol optimizes parameters of saliva collection/storage and DNA extraction to be of sufficient quality and quantity for DNA assays with the highest standards, including microarray genotyping and next generation sequencing.
摘要
在人类遗传学研究中，DNA的首选来源是血液或源自血液的细胞系，因为这些来源能产生大量高质量的DNA。然而，从唾液中提取DNA可以得到高质量的DNA，几乎没有降解/片段化，适用于各种DNA检测，而且无需采血技师，甚至可以通过邮寄获得。然而，目前文献中尚未出现用于下一代测序的唾液DNA收集/提取方案。本方案优化了唾液收集/储存和DNA提取的参数，以获得足够质量和数量的DNA用于最高标准的DNA检测，包括微阵列基因分型和下一代测序。
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