Whetsell W O, Allen G S, Tulipan N B
Department of Pathology, Vanderbilt University School of Medicine, Nashville, TN 37232.
Neurosci Lett. 1989 Jan 2;96(1):18-22. doi: 10.1016/0304-3940(89)90236-x.
Mature (greater than 21 days in vitro) organotypic corticostriatal cultures prepared from newborn rat brain were incubated in either kainic acid (KA) 10(-3) M or quinolinic acid (QUIN) 10(-3) M for up to 48 h. Other identical cultures were similarly incubated immediately after they had received one or two additional explants of neonatal striatal tissue placed beside each corticostriatal culture. The cultures incubated with either KA or QUIN in the presence of the neonatal striatal tissue showed better preservation than cultures incubated with KA or QUIN alone. Results suggest that the neonatal striatal explants or 'transplants' afford some protective effect against the toxicity or either KA or QUIN.
从新生大鼠大脑制备的成熟（体外培养超过21天）器官型皮质纹状体培养物在10⁻³ M的 kainic acid（KA）或10⁻³ M的喹啉酸（QUIN）中孵育长达48小时。其他相同的培养物在每个皮质纹状体培养物旁边放置一两个额外的新生纹状体组织外植体后立即进行类似孵育。在新生纹状体组织存在的情况下用KA或QUIN孵育的培养物比单独用KA或QUIN孵育的培养物保存得更好。结果表明，新生纹状体外植体或“移植体”对KA或QUIN的毒性具有一定的保护作用。
