Sudoh T, Maekawa K, Kojima M, Minamino N, Kangawa K, Matsuo H
Daiichi Pure Chemicals Co. Ltd., Tokyo, Japan.
Biochem Biophys Res Commun. 1989 Mar 31;159(3):1427-34. doi: 10.1016/0006-291x(89)92269-9.
Brain natriuretic peptide (BNP) is a novel diuretic-natriuretic and vasorelaxant peptide originally isolated from porcine brain. In contrast to mammalian atrial natriuretic peptide (ANP), immunological characterization suggests that mammalian BNPs show structural species differences. In order to determine the amino acid sequence of human BNP, we constructed a human cardiac atrium cDNA library and screened for clones hybridizing with porcine BNP cDNA. By sequence analysis of cDNA encoding a putative human BNP precursor, an amino acid sequence of human prepro-BNP of 134 residues has been deduced, in which a minimum bioactive unit highly homologous to porcine BNP-32 is present at the carboxy-terminus.
脑钠肽（BNP）是一种新型的利尿排钠和血管舒张肽，最初从猪脑中分离出来。与哺乳动物心房钠尿肽（ANP）不同，免疫特性表明哺乳动物BNP存在结构上的种属差异。为了确定人BNP的氨基酸序列，我们构建了人心脏心房cDNA文库，并筛选与猪BNP cDNA杂交的克隆。通过对编码假定的人BNP前体的cDNA进行序列分析，推导出了134个残基的人前脑钠肽原的氨基酸序列，其中在羧基末端存在一个与猪BNP-32高度同源的最小生物活性单位。
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