Romanenko Lyudmila A, Tanaka Naoto, Svetashev Vassilii I, Mikhailov Valery V
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Prospect 100 Let Vladivostoku, 159, 690022, Vladivostok, Russia,
Arch Microbiol. 2015 Mar;197(2):147-53. doi: 10.1007/s00203-014-1033-8. Epub 2014 Sep 17.
An aerobic, Gram-negative, yellow-pigmented, non-motile rod-shaped bacterium designated KMM 9574(T) was isolated from a sand sediment sample collected from the Sea of Japan seashore. Comparative 16S rRNA gene sequence analysis showed that strain KMM 9574(T) belonged to the genus Sphingorhabdus sharing a highest sequence similarity to Sphingorhabdus marina JCM 14161(T) 96.8 %. Strain KMM 9574(T) was characterized by the major ubiquinone Q-10, and by the predominance of C(18:1) ω7c, C(16:0) 2-OH, C(16:1) ω7c, C(17:1), followed by C(15:0) 2-OH and C(14:0) 2-OH in its fatty acid profile. Polar lipids consisted of phosphatidylcholine, sphingoglycolipid, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unknown phospholipid, and an unknown lipid. The DNA G+C content was 56.5 mol %. Based on phylogenetic analysis and distinctive phenotypic characteristics, strain 9574(T) is concluded to represent a novel species of the genus Sphingorhabdus, for which the name Sphingorhabdus pacificus sp. nov., is proposed. The type strain of the species is strain KMM 9574(T) (= NRIC 0922(T) = JCM 30177(T)).
从日本海沿岸采集的一份沙质沉积物样本中分离出一株需氧、革兰氏阴性、产黄色色素、无运动性的杆状细菌，命名为KMM 9574(T)。16S rRNA基因序列比较分析表明，菌株KMM 9574(T)属于鞘氨醇单胞菌属，与滨海鞘氨醇单胞菌JCM 14161(T)的序列相似性最高，为96.8%。菌株KMM 9574(T)的主要泛醌为Q-10，其脂肪酸谱以C(18:1)ω7c、C(16:0) 2-OH、C(16:1)ω7c、C(17:1)为主，其次是C(15:0) 2-OH和C(14:0) 2-OH。极性脂质由磷脂酰胆碱、鞘糖脂、磷脂酰乙醇胺、磷脂酰甘油、二磷脂酰甘油、一种未知磷脂和一种未知脂质组成。DNA G+C含量为56.5 mol%。基于系统发育分析和独特的表型特征，得出菌株9574(T)代表鞘氨醇单胞菌属的一个新物种的结论，为此提出太平洋鞘氨醇单胞菌（Sphingorhabdus pacificus）新种。该物种的模式菌株是菌株KMM 9574(T)（= NRIC 0922(T) = JCM 30177(T)）。
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