Romanenko Lyudmila A, Tanaka Naoto, Svetashev Vassilii I, Mikhailov Valery V
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Prospect 100 Let Vladivostoku, 159, Vladivostok, 690022, Russia,
Arch Microbiol. 2015 Jun;197(5):693-9. doi: 10.1007/s00203-015-1103-6. Epub 2015 Mar 19.
An aerobic, Gram-negative, motile, rod-shaped bacterium designated KMM 9500(T) was isolated from a sediment sample collected from the Sea of Japan seashore. Comparative 16S rRNA gene sequence analysis affiliated strain KMM 9500(T) to the genus Pseudomonas as a distinct subline clustered with Pseudomonas marincola KMM 3042(T) and Pseudomonas segetis KCTC 12331(T) sharing the highest similarities of 98 and 97.9 %, respectively. Strain KMM 9500(T) was characterized by mainly possessing ubiquinone Q-9, and by the predominance of C18:1 ω7c, C16:1 ω7c, and C16:0 followed by C12:0 in its fatty acid profile. Polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unknown aminophospholipid, and unknown phospholipids. Strain KMM 9500(T) was found to inhibit growth of Gram-negative and Gram-positive indicatory microorganisms. Based on the phylogenetic analysis and distinctive phenotypic characteristics, strain 9500(T) is concluded to represent a novel species of the genus Pseudomonas, for which the name Pseudomonas glareae sp. nov. is proposed. The type strain of the species is strain KMM 9500(T) (=NRIC 0939(T)).
从日本海沿岸采集的沉积物样本中分离出一株需氧、革兰氏阴性、具鞭毛、杆状的细菌，命名为KMM 9500(T)。通过比较16S rRNA基因序列分析，菌株KMM 9500(T)隶属于假单胞菌属，作为一个独特的亚分支，与海生假单胞菌KMM 3042(T)和草地假单胞菌KCTC 12331(T)聚类，相似性分别高达98%和97.9%。菌株KMM 9500(T)的特征主要在于主要含有泛醌Q-9，其脂肪酸谱以C18:1 ω7c、C16:1 ω7c和C16:0为主，其次是C12:0。极性脂质由磷脂酰乙醇胺、磷脂酰甘油、二磷脂酰甘油、一种未知氨基磷脂和未知磷脂组成。发现菌株KMM 9500(T)能抑制革兰氏阴性和革兰氏阳性指示微生物的生长。基于系统发育分析和独特的表型特征，得出菌株9500(T)代表假单胞菌属一个新物种的结论，为此提出新名称假单胞菌属格氏假单胞菌（Pseudomonas glareae sp. nov.）。该物种的模式菌株是菌株KMM 9500(T)（=NRIC 0939(T)）。
