Division of Applied Materials Science, Department of Engineering Sciences, Uppsala University, Sweden.
Department of Chemistry, BMC, Uppsala University, Sweden.
Acta Biomater. 2015 Jan;11:459-66. doi: 10.1016/j.actbio.2014.09.007. Epub 2014 Sep 16.
In this study a brushite cement was doped with the chain-breaking antioxidant Trolox. The effect of the antioxidant on the physical properties of the cement was evaluated and the release of Trolox was monitored by UV spectroscopy. The ability of the Trolox set free to scavenge reactive oxygen species (ROS) released by macrophages was determined in vitro using a luminol-amplified chemiluminescence assay. Trolox did not modify the crystalline phases of the set cement, which mainly formed crystalline brushite after 7 days in humid conditions. The setting time, compressive strength and morphology of the cement also remained unaltered after the addition of the antioxidant. Trolox was slowly released from the cement following a non-Fickian transport mechanism and nearly 64% of the total amount was released after 3 days. Moreover, the capacity of Trolox to scavenge the ROS released by macrophages increased in a dose-dependent manner. Trolox-loaded cements are expected to reduce some of the first harmful effects of acute inflammation and can thus potentially protect the surrounding tissue during implantation of these as well as other materials used in conjunction.
在这项研究中，我们在 brushite 水泥中掺杂了链断裂抗氧化剂 Trolox。通过紫外光谱法监测 Trolox 的释放，评估了抗氧化剂对水泥物理性能的影响。通过使用发光氨放大化学发光测定法，在体外确定了 Trolox 从水泥中释放出来后清除巨噬细胞释放的活性氧 (ROS) 的能力。Trolox 不会改变凝固水泥的晶体相，在潮湿条件下 7 天后，水泥主要形成结晶型 brushite。添加抗氧化剂后，水泥的凝固时间、抗压强度和形态仍保持不变。Trolox 以非菲克扩散机制从水泥中缓慢释放，3 天后几乎释放了 64%的总量。此外，Trolox 清除巨噬细胞释放的 ROS 的能力呈剂量依赖性增加。负载 Trolox 的水泥有望减轻急性炎症的一些最初的有害影响，因此在植入这些水泥以及与其他材料联合使用时，可以潜在地保护周围组织。
