Yang Y, Hu M, Zhang Y, Li H, Miao Z
Department of Medical Genetics, School of Basic Medical Sciences, Peking University, Beijing, 100191, China.
Cell Prolif. 2014 Dec;47(6):596-603. doi: 10.1111/cpr.12130. Epub 2014 Sep 18.
Human umbilical cord mesenchymal stem cells (hUCMSCs) play a critical role in expanding haematopoietic stem cells (HSCs) by providing the essential microenvironment for haematopoiesis. In this study, we sought to investigate whether CD29 of hUCMSCs would play a key role in the ability of hUCMSCs to help expand HSCs in vivo and in vitro.
To investigate whether CD29 of hUCMSCs would play a key role for the ability of hUCMSCs to expand HSCs, soluble anti-CD29 antibody was added to co-cultures of hUCMSCs and cord blood (CB) CD34(+) cells. It significantly blocked expansion of CB CD34(+) cells induced by hUCMSCs. Using CD29-deficient hUCMSCs models, long-term culture-initiating cell and non-obese diabetic/severe combined immunodeficient disease mouse repopulating cell assay, revealed that CB CD34(+) cells co-cultured with CD29-deficient hUCMSCs only retained the capacity of multipotent differentiation for 5 weeks at the most.
Soluble anti-CD29 antibody significantly blocked expansion of CB CD34(+) cells induced by hUCMSCs. CB CD34(+) cells co-cultured with CD29-deficient hUCMSCs only retained the capacity of multipotent differentiation for 5 weeks at the most.
CB CD34(+) cells co-cultured with CD29-deficient hUCMSCs gave rise to all major haematopoietic lineages, but failed to engraft long term.
人脐带间充质干细胞（hUCMSCs）通过为造血提供必要的微环境，在扩增造血干细胞（HSCs）中发挥关键作用。在本研究中，我们试图探究hUCMSCs的CD29是否在hUCMSCs体内外帮助扩增HSCs的能力中发挥关键作用。
为研究hUCMSCs的CD29是否在hUCMSCs扩增HSCs的能力中起关键作用，将可溶性抗CD29抗体添加到hUCMSCs与脐血（CB）CD34(+)细胞的共培养体系中。它显著阻断了hUCMSCs诱导的CB CD34(+)细胞的扩增。使用CD29缺陷的hUCMSCs模型，长期培养起始细胞和非肥胖糖尿病/重症联合免疫缺陷病小鼠再植细胞试验表明，与CD29缺陷的hUCMSCs共培养的CB CD34(+)细胞最多仅保留5周的多能分化能力。
可溶性抗CD29抗体显著阻断了hUCMSCs诱导的CB CD34(+)细胞的扩增。与CD29缺陷的hUCMSCs共培养的CB CD34(+)细胞最多仅保留5周的多能分化能力。
与CD29缺陷的hUCMSCs共培养的CB CD34(+)细胞可产生所有主要造血谱系，但未能长期植入。
