Zhang Yuanhao, Pan Xiuwei, Shi Zhen, Cai Haibo, Gao Yun, Zhang Weian
State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai, 200237, China.
Cell Prolif. 2018 Apr;51(2):e12407. doi: 10.1111/cpr.12407. Epub 2017 Nov 15.
Stem cell factor (SCF) is considered as a commonly indispensable cytokine for proliferation of haematopoietic stem cells (HSCs), which is used in large dosages during ex vivo culture. The work presented here aimed to reduce the consumption of SCF by sustained release but still support cells proliferation and maintain the multipotency of HSCs.
Stem cell factor was physically encapsulated within a hyaluronic acid/gelatin double network (HGDN) hydrogel to achieve a slow release rate. CD34 cells were cultured within the SCF-loaded HGDN hydrogel for 14 days. The cell number, phenotype and functional capacity were investigated after culture.
The HGDN hydrogels had desirable properties and encapsulated SCF kept being released for more than 6 days. SCF remained the native bioactivity, and the proliferation of HSCs within the SCF-loaded HGDN hydrogel was not affected, although the consumption of SCF was only a quarter in comparison with the conventional culture. Moreover, CD34 cells harvested from the SCF-loaded HGDN hydrogels generated more multipotent colony-forming units (CFU-GEMM).
The data suggested that the SCF-loaded HGDN hydrogel could support ex vivo culture of HSCs, thus providing a cost-effective culture protocol for HSCs.
干细胞因子（SCF）被认为是造血干细胞（HSCs）增殖普遍不可或缺的细胞因子，在体外培养过程中用量很大。本文介绍的工作旨在通过缓释减少SCF的消耗，但仍支持细胞增殖并维持造血干细胞的多能性。
将干细胞因子物理包裹在透明质酸/明胶双网络（HGDN）水凝胶中以实现缓慢释放速率。将CD34细胞在负载SCF的HGDN水凝胶中培养14天。培养后研究细胞数量、表型和功能能力。
HGDN水凝胶具有理想的特性，包裹的SCF持续释放超过6天。SCF保持天然生物活性，负载SCF的HGDN水凝胶中造血干细胞的增殖未受影响，尽管与传统培养相比SCF的消耗量仅为四分之一。此外，从负载SCF的HGDN水凝胶中收获的CD34细胞产生了更多的多能集落形成单位（CFU-GEMM）。
数据表明，负载SCF的HGDN水凝胶可支持造血干细胞的体外培养，从而为造血干细胞提供一种经济高效的培养方案。
