Chiu Nan-Fu, Huang Teng-Yi, Lai Hsin-Chih, Liu Kou-Chen
Institute of Electro-Optical Science and Technology, National Taiwan Normal University, No. 88, Sec. 4, Ting-Chou Road, Taipei 11677, Taiwan.
Department of Medical Biotechnology and Laboratory Science, Chang Gung University, No. 259 Wenhwa 1st Road, Gueishan Shiang, Taoyuan 33302, Taiwan.
Nanoscale Res Lett. 2014 Aug 28;9(1):445. doi: 10.1186/1556-276X-9-445. eCollection 2014.
This work develops a highly sensitive immunoassay sensor for use in graphene oxide sheet (GOS)-based surface plasmon resonance (SPR) chips. This sensing film, which is formed by chemically modifying a GOS surface, has covalent bonds that strongly interact with the bovine serum albumin (BSA), explaining why it has a higher sensitivity. This GOS film-based SPR chip has a BSA concentration detection limit that is 100 times higher than that of the conventional Au-film-based sensor. The affinity constants (K A) on the GOS film-based SPR chip and the conventional SPR chip for 100 μg/ml BSA are 80.82 × 10(6) M(-1) and 15.67 × 10(6) M(-1), respectively. Therefore, the affinity constant of the GOS film-based SPR chip is 5.2 times higher than that of the conventional chip. With respect to the protein-protein interaction, the SPR sensor capability to detect angle changes at a low concentration anti-BSA of 75.75 nM on the GOS film-based SPR chip and the conventional SPR chip is 36.1867 and 26.1759 mdeg, respectively. At a high concentration, anti-BSA of 378.78 nM on the GOS film-based SPR chip and the conventional SPR chip reveals two times increases in the SPR angle shift. Above results demonstrate that the GOS film is promising for highly sensitive clinical diagnostic applications.
这项工作开发了一种用于基于氧化石墨烯片（GOS）的表面等离子体共振（SPR）芯片的高灵敏度免疫分析传感器。这种传感膜通过对GOS表面进行化学修饰形成，具有与牛血清白蛋白（BSA）强烈相互作用的共价键，这解释了其为何具有更高的灵敏度。这种基于GOS膜的SPR芯片的BSA浓度检测限比传统的基于金膜的传感器高100倍。基于GOS膜的SPR芯片和传统SPR芯片对100μg/ml BSA的亲和常数（KA）分别为80.82×10(6) M(-1)和15.67×10(6) M(-1)。因此，基于GOS膜的SPR芯片的亲和常数比传统芯片高5.2倍。关于蛋白质 - 蛋白质相互作用，基于GOS膜的SPR芯片和传统SPR芯片在75.75 nM低浓度抗BSA下检测角度变化的SPR传感器能力分别为36.1867和26.1759 mdeg。在高浓度下，基于GOS膜的SPR芯片和传统SPR芯片上378.78 nM的抗BSA显示SPR角移增加了两倍。上述结果表明，GOS膜在高灵敏度临床诊断应用方面具有前景。
