Grimm Martin, Cetindis Marcel, Lehmann Max, Biegner Thorsten, Munz Adelheid, Teriete Peter, Reinert Siegmar
Department of Oral and Maxillofacial Surgery.
Acta Odontol Scand. 2015 Jul;73(5):336-42. doi: 10.3109/00016357.2014.961029. Epub 2014 Sep 19.
Apoptosis resistance is a crucial factor for the carcinogenesis of oral squamous cell carcinoma (OSCC).
Expression of apoptosis resistance-related ATP-binding cassette (ABC) transporter ABCB5 [subfamily B (MDR/TAP) member 5] and DNaseX (Apo10) were analyzed in normal oral mucosa (n = 5), oral precursor lesions (simple hyperplasia, n = 11; squamous intraepithelial neoplasia, SIN I-III, n = 35), and OSCC specimen (n = 42) by immunohistochemistry.
Expression of ABCB5 and Apo10 were significantly increased in the carcinogenesis of OSCC compared with normal tissue. Compared with SIN I-III, ABCB5 expression was significantly decreased in OSCC. Apo10 expression did not significantly differ from OSCC compared with SIN I-III.
This study provides the first evidence of the expression of ABCB5 and Apo10 in the multi-step carcinogenesis of OSCC. Overcoming drug resistance of ABCB5+ and Apo10+ cells in precursor lesions and tumors by natural compounds may act as sensitizers for apoptosis or could be useful for chemoprevention.
凋亡抗性是口腔鳞状细胞癌（OSCC）致癌作用的关键因素。
通过免疫组织化学分析凋亡抗性相关的ATP结合盒（ABC）转运蛋白ABCB5[B亚家族（MDR/TAP）成员5]和脱氧核糖核酸酶X（Apo10）在正常口腔黏膜（n = 5）、口腔前驱病变（单纯增生，n = 11；鳞状上皮内瘤变，SIN I - III，n = 35）以及OSCC标本（n = 42）中的表达。
与正常组织相比，ABCB5和Apo10在OSCC致癌过程中的表达显著增加。与SIN I - III相比，ABCB5在OSCC中的表达显著降低。与SIN I - III相比，Apo10在OSCC中的表达无显著差异。
本研究首次提供了ABCB5和Apo10在OSCC多步骤致癌过程中表达的证据。通过天然化合物克服前驱病变和肿瘤中ABCB5+和Apo10+细胞的耐药性，可能作为凋亡的敏化剂或对化学预防有用。
