Niimura S, Mizuno K, Yabe R, Tani M, Suenaga K, Naruse M, Inagami T, Fukuchi S
Department of Internal Medicine III, Fukushima Medical College, Japan.
Res Commun Chem Pathol Pharmacol. 1989 Feb;63(2):189-200.
To clarify the presence of atrial natriuretic peptide (ANP) in neural tissue, extracts from human neuroblastoma which is considered of neural crest origin were analyzed using a specific radioimmunoassay (RIA) for ANP. High concentrations of immunoreactive ANP ranging from 2.7 to 18.4 ng per mg of protein were demonstrated in the tissue. Furthermore, high performance gel permeation chromatography (HPGPC) coupled with the RIA revealed that the immunoreactive ANP found in the tissue consisted of only one component with molecular weight of 12,000 to 13,000 daltons, corresponding to gamma-human ANP (hANP). These results could be direct evidence for generation of ANP intrinsic of human neuronal tissue, and also suggest that neuroblastoma can be used as a model for investigation of mechanism of ANP formation within the neuronal tissues.
为了阐明神经组织中是否存在心钠素（ANP），我们使用针对ANP的特异性放射免疫分析法（RIA），对源自神经嵴的人神经母细胞瘤提取物进行了分析。该组织中显示出高浓度的免疫反应性ANP，每毫克蛋白质的含量在2.7至18.4纳克之间。此外，高效凝胶渗透色谱法（HPGPC）结合RIA显示，该组织中发现的免疫反应性ANP仅由一种分子量为12,000至13,000道尔顿的成分组成，对应于γ-人ANP（hANP）。这些结果可能是人类神经元组织内源性产生ANP的直接证据，也表明神经母细胞瘤可作为研究神经元组织内ANP形成机制的模型。
