人和大鼠心钠素及其前体。

Human and rat atrial natriuretic polypeptides and their precursors.

作者信息

Matsuo H, Kangawa K

出版信息

J Hypertens Suppl. 1986 Oct;4(3):S299-302.

Abstract

A highly sensitive radio-immunoassay (RIA) to detect all the atrial natriuretic polypeptides (ANPs) isolated so far from human and rat (hANPs and rANPs) has been established by using an antiserum raised against alpha-hANP. The antiserum recognizes the sub-sequence flanked by two cysteine residues (positions 7 and 23) in alpha-hANP and cross-reacts with hANPs as well as rANPs. By using the RIA combined with gel chromatography or high-performance liquid chromatography, it was revealed that ANP immunoreactivity in human atria is composed of alpha- (28 residues), beta- (56 residues: alpha-hANP dimer) and gamma-hANP (126 residues) in various ratios. In rat, porcine and bovine atrial tissues, however, gamma-rANP (126 residues) was found to be a major component. In contrast with atrial ANP, plasma ANP was found to be predominantly alpha-form in rat.

摘要

通过使用针对α-人心房利钠多肽（α-hANP）产生的抗血清，建立了一种高灵敏度放射免疫分析法（RIA），用于检测迄今为止从人和大鼠中分离出的所有心房利钠多肽（hANPs和rANPs）。该抗血清识别α-hANP中由两个半胱氨酸残基（第7和23位）侧翼的亚序列，并与hANPs以及rANPs发生交叉反应。通过将RIA与凝胶色谱或高效液相色谱相结合使用，发现人心房中的ANP免疫反应性由α-（28个残基）、β-（56个残基：α-hANP二聚体）和γ-hANP（126个残基）以不同比例组成。然而，在大鼠、猪和牛的心房组织中，γ-rANP（126个残基）被发现是主要成分。与心房ANP相反，在大鼠中血浆ANP主要是α形式。