Ationu A, Carter N D
Department of Cardiac Surgery, Harefield Hospital, Middlesex, England.
Br J Biomed Sci. 1994 Dec;51(4):316-20.
We examined the molecular forms of brain natriuretic peptide (BNP) and atrial natriuretic peptide (ANP) in endomyocardial right ventricular extracts from cardiac transplant recipients, using gel chromatographic separation coupled with specific radioimmunoassay techniques. Two distinct molecular forms of ANP and BNP were observed in the ventricular extracts consistent with the biologically active alpha-human peptides and the gamma-precursor peptides. Alpha-BNP was predominant and constituted 75 +/- 3% (n = 5) of the total immunoreactive BNP, while 21 +/- 2% (n = 5) of the total immunoreactivity comprised gamma-BNP. In contrast, gamma-ANP constituted 89 +/- 4% (n = 5) of the total immunoreactive ANP and was dominant over alpha-ANP, which made up 10 +/- 2% (n = 5) of the total immunoreactivity. There was no evidence for altered processing of BNP and ANP precursors in the right ventricular tissues from the de-nervated transplanted human heart. These findings provide further insight by identifying the molecular forms of BNP and ANP present in the transplanted human heart.
我们采用凝胶色谱分离结合特异性放射免疫分析技术，检测了心脏移植受者右心室心内膜提取物中脑钠肽（BNP）和心房钠尿肽（ANP）的分子形式。在心室提取物中观察到两种不同的ANP和BNP分子形式，与具有生物活性的α-人肽和γ-前体肽一致。α-BNP占主导地位，占总免疫反应性BNP的75±3%（n = 5），而γ-BNP占总免疫反应性的21±2%（n = 5）。相比之下，γ-ANP占总免疫反应性ANP的89±4%（n = 5），且比α-ANP占优势，α-ANP占总免疫反应性的10±2%（n = 5）。没有证据表明去神经支配的移植人心脏右心室组织中BNP和ANP前体的加工过程发生改变。这些发现通过鉴定移植人心脏中存在的BNP和ANP分子形式，提供了进一步的见解。
