State Key Joint Laboratory of Environmental Simulation and Pollution Control (Peking University), College of Environmental Sciences and Engineering, Peking University, Beijing, 100871, China.
Appl Microbiol Biotechnol. 2015 Feb;99(4):1935-46. doi: 10.1007/s00253-014-6074-z. Epub 2014 Sep 20.
Alkanes and polycyclic aromatic hydrocarbons (PAHs) are the commonly detected petroleum hydrocarbon contaminants in soils in oil exploring areas. Hydrocarbon-degrading genes are useful biomarks for estimation of the bioremediation potential of contaminated sites. However, the links between environmental factors and the distribution of alkane and PAH metabolic genes still remain largely unclear. The present study investigated the abundances and diversities of soil n-alkane and PAH-degrading bacterial communities targeting both alkB and nah genes in two oil exploring areas at different geographic regions. A large variation in the abundances and diversities of alkB and nah genes occurred in the studied soil samples. Various environmental variables regulated the spatial distribution of soil alkane and PAH metabolic genes, dependent on geographic location. The soil alkane-degrading bacterial communities in oil exploring areas mainly consisted of Pedobacter, Mycobacterium, and unknown alkB-harboring microorganisms. Moreover, the novel PAH-degraders predominated in nah gene clone libraries from soils of the two oil exploring areas. This work could provide some new insights towards the distribution of hydrocarbon-degrading microorganisms and their biodegradation potential in soil ecosystems.
烷烃和多环芳烃（PAHs）是石油勘探区土壤中常见的石油烃污染物。烃降解基因是评估污染场地生物修复潜力的有用生物标志物。然而，环境因素与烷烃和 PAH 代谢基因分布之间的联系在很大程度上仍不清楚。本研究调查了两个不同地理位置的石油勘探区土壤中 n-烷烃和 PAH 降解细菌群落针对 alkB 和 nah 基因的丰度和多样性。在所研究的土壤样本中，alkB 和 nah 基因的丰度和多样性存在很大差异。各种环境变量根据地理位置调节土壤烷烃和 PAH 代谢基因的空间分布。石油勘探区土壤烷烃降解细菌群落主要由 Pedobacter、Mycobacterium 和未知的 alkB 携带微生物组成。此外，来自两个石油勘探区土壤的 nah 基因克隆文库中主要存在新型 PAH 降解菌。这项工作可以为烃降解微生物的分布及其在土壤生态系统中的生物降解潜力提供一些新的见解。
