Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin 300072, China.
SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
Int J Environ Res Public Health. 2020 Mar 2;17(5):1606. doi: 10.3390/ijerph17051606.
Due to the increasing pollution by petroleum hydrocarbons (PHs), it is an important task to develop eco-friendly and highly efficient methods for remediating petroleum-contaminated soils. In this study, bioremediation technology was applied to remediate PHs contaminated soils, and the bacterial community structure and physicochemical characteristics of the soil treated using different bioremediation regimens were analyzed. Compared with the control condition (S0), the PHs removal efficiency of biostimulation (S2) and bioaugmentation (S3) was increased significantly. Combined biostimulation with bioaugmentation (S4) had the highest PHs removal efficiency, up to 60.14 ± 4.12%. Among all the selected remediation strategies (S1-S4, S1: soil moisture content: 25-30%), the bacterial alpha-diversity was higher than in S0. The genera , , , , and were found to greatly contribute to PHs' degradation. In the group S4, the PH-degraders and soil enzyme activity were higher than in the other remediation regimens, and these indices gradually decreased in the mid-to-later periods of all remediation tests. Additionally, the abundance of and genes was increased by improving the environmental condition of the microorganism communities. Redundancy analysis (RDA) revealed that the total nitrogen (TN) and total phosphorus (TP) had a positive correlation with total PHs degradation. This study offers insights into the microbial community response to environmental factors during bioremediation, which shows a promoting effect in enhancing the efficiency of PHs remediation.
由于石油烃（PHs）污染的增加，开发环保且高效的修复受石油污染土壤的方法是一项重要任务。本研究应用生物修复技术修复 PHs 污染土壤，分析了不同生物修复方案处理后土壤的细菌群落结构和理化特性。与对照条件（S0）相比，生物刺激（S2）和生物强化（S3）的 PHs 去除效率显著提高。生物刺激与生物强化相结合（S4）的 PHs 去除效率最高，达到 60.14±4.12%。在所有选择的修复策略（S1-S4，S1：土壤含水量：25-30%）中，细菌α多样性高于 S0。发现 、 、 、 和 等属对 PHs 的降解有很大贡献。在 S4 组中，PH 降解菌和土壤酶活性高于其他修复方案，并且所有修复试验的中后期这些指标逐渐降低。此外，通过改善微生物群落的环境条件，增加了 和 基因的丰度。冗余分析（RDA）表明，总氮（TN）和总磷（TP）与总 PHs 降解呈正相关。本研究深入了解了微生物群落对生物修复过程中环境因素的响应，这表明在提高 PHs 修复效率方面具有促进作用。
