二年生艾蒿地上部分不同提取物和馏分的总酚含量及抗氧化活性
Total Phenolic Contents and Antioxidant Activities of Different Extracts and Fractions from the Aerial Parts of Artemisia biennis Willd.
作者信息
Hatami Tayyebe, Emami Sayyed Ahmad, Miraghaee Sayyed Shahram, Mojarrab Mahdi
机构信息
Novel Drug Delivery Research Center, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran. ; Student Research Committee, Kermanshah University of Medical Science, Kermanshah, Iran.
Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
出版信息
Iran J Pharm Res. 2014 Spring;13(2):551-9.
Total phenolic contents (TPC) of five different extracts (petroleum ether, dichloromethane, ethyl acetate, ethanol and ethanol-water) of Artemisia biennis Willd were measured in this work. The antioxidant activity was investigated by three different methods: β-carotene bleaching (BCB) test, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method and ferrous ion chelating (FIC) assay. Among all the extracts analyzed, the hydroethanolic extract exhibited a significantly higher phenolic content and antioxidant activity than other samples. Vacuum liquid chromatography of this extract yielded seven fractions (A to G) which were subjected to all aforementioned experiments. The highest total phenolic content and free radical scavenging activites were present in the same sample (Fraction D) but the only statistically significant correlation between TPC and EC50 values was observed for BCB.
本研究测定了二年生蒿（Artemisia biennis Willd）五种不同提取物（石油醚、二氯甲烷、乙酸乙酯、乙醇和乙醇 - 水）的总酚含量（TPC）。通过三种不同方法研究抗氧化活性：β - 胡萝卜素漂白（BCB）试验、2,2 - 二苯基 - 1 - 苦基肼（DPPH）自由基清除法和亚铁离子螯合（FIC）测定法。在所有分析的提取物中，乙醇 - 水提取物表现出比其他样品显著更高的酚含量和抗氧化活性。该提取物的真空液相色谱产生了七个馏分（A至G），对其进行了上述所有实验。最高的总酚含量和自由基清除活性存在于同一样品（馏分D）中，但仅在BCB中观察到TPC与EC50值之间具有统计学意义的相关性。
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