Caniatti Marcela Caleffi da Costa Lima, Marchioro Ariella Andrade, Guilherme Ana Lúcia Falavigna, Tsuneto Luiza Tamie
Post-Graduate Program in Health Sciences, Universidade Estadual de Maringá (UEM), Maringá, Paraná, Brazil.
Department of Basic Health Sciences, UEM, Maringá, Paraná, Brazil.
PLoS One. 2014 Sep 19;9(9):e107921. doi: 10.1371/journal.pone.0107921. eCollection 2014.
Allergic reaction to dust mites is a relatively common condition among children, triggering cutaneous and respiratory responses that have a great impact on the health of this population. Anaphylactic hypersensitivity is characterized by an exacerbated response involving the production of regulatory cytokines responsible for stimulating the production of IgE antibodies.
To investigate an association of variants in cytokine genes (IL1A-889, IL1B-511, +3962, IL1R1970, IL1RA11100, IL4RA+1902, IL12-1188, IFNG+874, TGFB1 codon 10, codon 25, TNFA-308, -238, IL2-330, +166, IL4-1098, -590, -33, IL6-174, nt565, and IL10-1082, -819, -592) between patients sensitive to dust mites and a control group.
A total of 254 patients were grouped as atopic and non-atopic according to sensitivity as evaluated by the Prick Test and to cytokine genotyping by the polymerase chain reaction-sequence specific primers (PCR-SSP) method using the Cytokine Genotyping Kit.
A comparison between individuals allergic to Dermatophagoides farinae, Dermatophagoides pteronyssinus, and Blomia tropicalis and a non-atopic control group showed significant differences between allele and genotype frequencies in the regulatory regions of cytokine genes, with important evidence for IL4-590 in T/C (10.2% vs. 43.1%, odd ratio [OR] = 0.15, p = 5.2 10-8, pc = 0.0000011, and 95% confidence interval [95%CI] = 0.07-0.32) and T/T genotypes (42.9% vs. 13.8%, OR = 4.69, p = 2.5 10-6, pc = 0.000055, and 95%CI = 2.42-9.09). Other associations were observed in the pro-inflammatory cytokines IL1A-889 (T/T, C, and T) and IL2-330 (G/T and T/T) and the anti-inflammatory cytokines IL4RA+1902 (A and G), IL4-590 (T/C, T/T, C, and T), and IL10-592 (A/A, C/A, A, and C).
Our results suggest a possible association between single nucleotide polymorphisms (SNPs) in cytokine genes and hypersensitivity to dust mites.
对尘螨的过敏反应在儿童中较为常见，会引发皮肤和呼吸道反应，对这一群体的健康产生重大影响。过敏反应性超敏反应的特征是反应加剧，涉及产生负责刺激IgE抗体产生的调节性细胞因子。
研究细胞因子基因（IL1A - 889、IL1B - 511、+3962、IL1R1 970、IL1RA 11100、IL4RA +1902、IL12 - 1188、IFNG +874、TGFB1密码子10、密码子25、TNFA - 308、 - 238、IL2 - 330、+166、IL4 - 1098、 - 590、 - 33、IL6 - 174、nt565以及IL10 - 1082、 - 819、 - 592）的变异在尘螨敏感患者与对照组之间的关联。
根据点刺试验评估的敏感性以及使用细胞因子基因分型试剂盒通过聚合酶链反应 - 序列特异性引物（PCR - SSP）方法进行的细胞因子基因分型，将254例患者分为特应性和非特应性两组。
对粉尘螨、屋尘螨和热带无爪螨过敏的个体与非特应性对照组之间的比较显示，细胞因子基因调控区域的等位基因和基因型频率存在显著差异，IL4 - 590的T/C（10.2%对43.1%，优势比[OR]=0.15，p = 5.2×10 - 8，pc = 0.0000011，95%置信区间[95%CI]=0.07 - 0.32）和T/T基因型（42.9%对13.8%，OR = 4.69，p = 2.5×10 - 6，pc = 0.000055，95%CI = 2.42 - 9.09）有重要证据。在促炎细胞因子IL1A - 889（T/T、C和T）和IL2 - 330（G/T和T/T）以及抗炎细胞因子IL4RA +1902（A和G）、IL4 - 590（T/C、T/T、C和T）和IL10 - 592（A/A、C/A、A和C）中观察到其他关联。
我们的结果表明细胞因子基因中的单核苷酸多态性（SNP）与对尘螨的超敏反应之间可能存在关联。
