白细胞介素 10（IL10）和转化生长因子β1（TGFβ1）基因多态性与持续性 IgE 介导的牛奶过敏
Interleukin 10 (IL10) and transforming growth factor β1 (TGFβ1) gene polymorphisms in persistent IgE-mediated cow's milk allergy.
机构信息
Allergy and Immunology Unit, Department of Pediatrics, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brazil.
出版信息
Clinics (Sao Paulo). 2013 Jul;68(7):1004-9. doi: 10.6061/clinics/2013(07)19.
OBJECTIVES
The aim of this cross-sectional study was to evaluate whether interleukin 10 (IL10) and transforming growth factor β1 (TGFβ1) gene polymorphisms were associated with persistent IgE-mediated cow's milk allergy in 50 Brazilian children. The diagnostic criteria were anaphylaxis triggered by cow's milk or a positive double-blind, placebo-controlled food challenge. Tolerance was defined as the absence of a clinical response to a double-blind, placebo-controlled food challenge or cow's milk exposure.
METHOD
The genomic DNA of the 50 patients and 224 healthy controls (HCs) was used to investigate five IL10 gene polymorphisms (-3575A/T, -2849A/G, -2763A/C, -1082G/A, -592C/A) and one TGFβ1 polymorphism (-509C/T).
RESULTS
Among the five IL10 polymorphisms analyzed, homozygosis for the G allele at the -1082 position was significantly higher in the patients compared with the healthy controls (p=0.027) and in the persistent cow's milk allergy group compared with the healthy controls (p=0.001).
CONCLUSIONS
Homozygosis for the G allele at the IL10 -1082G/A polymorphism is associated with the persistent form of cow's milk allergy.
目的
本横断面研究旨在评估白细胞介素 10（IL10）和转化生长因子β1（TGFβ1）基因多态性是否与 50 名巴西儿童持续性 IgE 介导的牛奶过敏有关。诊断标准为牛奶触发的过敏反应或双盲、安慰剂对照食物挑战阳性。耐受性定义为对双盲、安慰剂对照食物挑战或牛奶暴露无临床反应。
方法
使用 50 名患者和 224 名健康对照者（HCs）的基因组 DNA 来研究 5 个 IL10 基因多态性（-3575A/T、-2849A/G、-2763A/C、-1082G/A、-592C/A）和 1 个 TGFβ1 多态性（-509C/T）。
结果
在分析的 5 个 IL10 多态性中，与健康对照组相比，患者中 -1082 位置的 G 等位基因纯合子明显更高（p=0.027），与健康对照组相比，持续性牛奶过敏组的 G 等位基因纯合子更高（p=0.001）。
结论
IL10-1082G/A 多态性的 G 等位基因纯合子与牛奶过敏的持续性形式有关。
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