Stem Cell Research Center, Taipei Medical University, Taipei, Taiwan, ROC; Graduate Institute of Biomedical Materials and Tissue Engineering, Taipei Medical University, Taipei, Taiwan, ROC.
Department of Dentistry, Taipei Medical University Hospital, Taipei, Taiwan, ROC.
Biomaterials. 2014 Dec;35(37):9767-9776. doi: 10.1016/j.biomaterials.2014.08.034. Epub 2014 Sep 16.
Aging is related to loss of functional stem cell accompanying loss of tissue and organ regeneration potentials. Previously, we demonstrated that the life span of ovariectomy-senescence accelerated mice (OVX-SAMP8) was significantly prolonged and similar to that of the congenic senescence-resistant strain of mice after platelet rich plasma (PRP)/embryonic fibroblast transplantation. The aim of this study is to investigate the potential of PRP for recovering cellular potential from senescence and then delaying animal aging. We first examined whether stem cells would be senescent in aged mice compared to young mice. Primary adipose derived stem cells (ADSCs) and bone marrow derived stem cells (BMSCs) were harvested from young and aged mice, and found that cell senescence was strongly correlated to animal aging. Subsequently, we demonstrated that PRP could recover cell potential from senescence, such as promote cell growth (cell proliferation and colony formation), increase osteogenesis, decrease adipogenesis, restore cell senescence related markers and resist the oxidative stress in stem cells from aged mice. The results also showed that PRP treatment in aged mice could delay mice aging as indicated by survival, body weight and aging phenotypes (behavior and gross morphology) in term of recovering the cellular potential of their stem cells compared to the results on aged control mice. In conclusion these findings showed that PRP has potential to delay aging through the recovery of stem cell senescence and could be used as an alternative medicine for tissue regeneration and future rejuvenation.
衰老是与功能干细胞的丧失有关的，而功能干细胞的丧失伴随着组织和器官再生潜能的丧失。以前，我们证明了富血小板血浆（PRP）/胚胎成纤维细胞移植后，卵巢切除加速衰老的小鼠（OVX-SAMP8）的寿命明显延长，与同源的衰老抗性品系小鼠的寿命相似。本研究的目的是探讨 PRP 恢复衰老细胞潜能并延缓动物衰老的潜力。我们首先研究了与年轻小鼠相比，衰老小鼠中的干细胞是否会衰老。从小鼠和老年鼠中分离出原代脂肪来源干细胞（ADSCs）和骨髓来源干细胞（BMSCs），发现细胞衰老与动物衰老密切相关。随后，我们证明了 PRP 可以从衰老中恢复细胞潜能，例如促进细胞生长（细胞增殖和集落形成）、增加成骨作用、减少成脂作用、恢复细胞衰老相关标志物并抵抗衰老小鼠干细胞中的氧化应激。结果还表明，与衰老对照小鼠相比，PRP 处理衰老小鼠可以通过恢复其干细胞的细胞潜能来延缓小鼠衰老，这表现在存活、体重和衰老表型（行为和大体形态）方面。总之，这些发现表明 PRP 通过恢复干细胞衰老具有延缓衰老的潜力，可作为组织再生和未来抗衰老的替代疗法。
