Zou Jun, Yuan Chenxi, Wu Chunshen, Cao Cheng, Yang Huilin
Department of Orthopaedic Surgery, The First Affiliated Hospital of Soochow University , Suzhou, Jiangsu , China.
Connect Tissue Res. 2014 Aug;55(4):304-9. doi: 10.3109/03008207.2014.930140. Epub 2014 Jun 18.
Bone marrow mesenchymal stem cells (BMSCs) are multipotent stem cells. Finding methods to improve the osteogenic potential of these cells is a key factor in bone tissue engineering. Platelet-rich plasma (PRP) contains powerful growth factors that produce changes in a variety of cell types. The purpose of this study was to explore the effects of PRP on the osteogenic differentiation of BMSCs in vitro. Rabbit BMSCs were harvested and cultured in vitro in control media or in media enhanced with PRP. BMSCs began to attach 12-24 hours after seeding. A MTT assay demonstrated that PRP-induced BMSCs grew rapidly compared with the control group. The PRP group also showed strongly positive staining of alkaline phosphatase and mineralized nodules whereas the control group showed negative staining. However, the alkaline phosphatase activity and the mRNA level of the osteogenic markers (osteocalcin and osteopontin) remained higher in the PRP group. These results confirmed that PRP could enhance the proliferation of BMSCs and effectively promote the osteogenic differentiation of BMSCs in vitro.
骨髓间充质干细胞（BMSCs）是多能干细胞。找到提高这些细胞成骨潜能的方法是骨组织工程的关键因素。富血小板血浆（PRP）含有强大的生长因子，可使多种细胞类型发生变化。本研究的目的是探讨PRP对体外培养的BMSCs成骨分化的影响。收获兔BMSCs并在对照培养基或添加PRP的培养基中进行体外培养。接种后12 - 24小时，BMSCs开始贴壁。MTT分析表明，与对照组相比，PRP诱导的BMSCs生长迅速。PRP组碱性磷酸酶和矿化结节染色也呈强阳性，而对照组染色呈阴性。然而，PRP组碱性磷酸酶活性和成骨标志物（骨钙素和骨桥蛋白）的mRNA水平仍然较高。这些结果证实，PRP可增强BMSCs的增殖，并有效促进体外培养的BMSCs的成骨分化。
