Stashenko P, Yu S M
Department of Immunology, Forsyth Dental Center, Boston, Massachusetts 02115.
J Dent Res. 1989 May;68(5):830-4. doi: 10.1177/00220345890680051601.
T helper (TH) and T suppressor (TS) cells were enumerated in developing rat periapical lesions in order to identify the cell type associated with the active phase of lesion formation. Experimental pulp exposures were made in all molar teeth of 29 Sprague-Dawley rats, and teeth were left open to the oral environment. On days 15, 20, 30, and 90 after induction, inflammatory cells were isolated from periapical lesions, and T helper (W3/25+), T suppressor (OX8+), and total T cells (W3/13+) were enumerated by reactivity with monoclonal antibodies. During the early, most active phase of lesion development (day 15), TH cells outnumbered TS cells (TH/TS = 1.7). However, by day 20, when lesion expansion had slowed, the TH/TS ratio was reversed (0.9) and remained so for up to 90 days after induction (TH/TS = 0.7). Total T cell numbers did not change over the period of observation. TH cells thus predominated during the active phase of lesion development, whereas TS cells were associated with the chronic phase. This dynamic TH/TS reversal indicates that immune responses which occur within periapical lesions are highly regulated. In addition, the predominance of TH during the active phase of lesion development suggests that TH-mediated activities may be involved in the bone destruction and immune cell activation within periapical lesions.
为了确定与根尖周病变形成活跃期相关的细胞类型，对发育中的大鼠根尖周病变中的辅助性T细胞（TH）和抑制性T细胞（TS）进行了计数。在29只Sprague-Dawley大鼠的所有磨牙上进行实验性牙髓暴露，并使牙齿向口腔环境开放。在诱导后的第15、20、30和90天，从根尖周病变中分离出炎性细胞，通过与单克隆抗体的反应性对辅助性T细胞（W3/25+）、抑制性T细胞（OX8+）和总T细胞（W3/13+）进行计数。在病变发展的早期、最活跃阶段（第15天），TH细胞数量超过TS细胞（TH/TS = 1.7）。然而，到第20天，当病变扩展减缓时，TH/TS比值发生逆转（0.9），并且在诱导后长达90天内一直保持这种状态（TH/TS = 0.7）。在观察期内总T细胞数量没有变化。因此，TH细胞在病变发展的活跃期占主导，而TS细胞与慢性期相关。这种动态的TH/TS逆转表明根尖周病变内发生的免疫反应受到高度调节。此外，TH在病变发展活跃期的优势表明TH介导的活动可能参与根尖周病变内的骨质破坏和免疫细胞激活。
