Rezende Taia Maria Berto, Vieira Leda Quercia, Sobrinho Antônio Paulino Ribeiro, Oliveira Ricardo Reis, Taubman Martin A, Kawai Toshihisa
Departamento de Dentística Restauradora, Faculdade de Odontologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
J Endod. 2008 Sep;34(9):1066-71. doi: 10.1016/j.joen.2008.06.006.
This study assessed the influence of mineral trioxide aggregate (MTA) on adaptive immune responses. BALB/c mice were immunized with heat-killed Fusobacterium nucleatum (Fn) in MTA or other control adjuvants, and serum IgG responses to Fn were measured. Either Fn- or Peptostreptococcus anaerobius (Pa)-reactive memory T cells (Tm) were preincubated in vitro with/without MTA and restimulated with Fn or Pa. Tm proliferation and cytokine production were assessed. Compared with control groups, immunoglobulin G-antibody responses were upregulated in mice immunized with Fn in MTA in a similar manner to animals immunized with Fn in Freund's adjuvant or aluminum hydroxide adjuvant. Although MTA did not affect the upregulated expression of interleukin 10, tumor necrosis factor alpha, or RANKL by Tm, it suppressed the proliferation of Pa- or Fn-Tm and inhibited their production of Th1- or Th2-signature cytokines. MTA upregulated the adaptive humoral immune responses but had little or no effect on pro- or anti-inflammatory cytokine production by Tm.
本研究评估了三氧化矿物凝聚体（MTA）对适应性免疫反应的影响。将BALB/c小鼠用热灭活的具核梭杆菌（Fn）在MTA或其他对照佐剂中进行免疫，并检测血清中对Fn的IgG反应。将Fn或厌氧消化链球菌（Pa）反应性记忆T细胞（Tm）在体外与MTA一起或不与MTA一起预孵育，并用Fn或Pa再次刺激。评估Tm的增殖和细胞因子产生。与对照组相比，在用MTA中的Fn免疫的小鼠中，免疫球蛋白G抗体反应以与用弗氏佐剂或氢氧化铝佐剂中的Fn免疫的动物相似的方式上调。尽管MTA不影响Tm对白介素10、肿瘤坏死因子α或RANKL的上调表达，但它抑制了Pa或Fn-Tm的增殖，并抑制了它们产生Th1或Th2标志性细胞因子。MTA上调了适应性体液免疫反应，但对Tm产生的促炎或抗炎细胞因子几乎没有影响。
