Neijat M, Gakhar N, Neufeld J, House J D
Department of Animal Science, University of Manitoba, Winnipeg, Manitoba, MB, R3T 2N2, Canada.
Department of Animal Science, University of Manitoba, Winnipeg, Manitoba, MB, R3T 2N2, Canada Department of Human Nutritional Sciences, University of Manitoba, Winnipeg, Manitoba, MB, R3T 2N2, Canada
Poult Sci. 2014 Nov;93(11):2827-40. doi: 10.3382/ps.2014-03936. Epub 2014 Sep 19.
The aim of this study was to compare the performance of hens (feed intake, rate of lay, egg weight, and BW gain), egg quality and blood biochemistry (enzymes, electrolytes, proteins, and other plasma constituents) of laying hens fed diets containing hemp products. Forty-eight Lohmann LSL-Classic (white-egg layers; 19 wk of age) were individually caged and fed 1 of 6 wheat-barley-soybean-based diets for a period of 12 wk. The diets consisted of hempseed (HS; 10, 20, or 30%), hempseed oil (HO; 4.5 or 9.0%), or a control diet (corn oil-based). All diets were formulated to contain similar levels of crude fat (11%), energy (2,800 kcal/kg), and CP (17%). Data were analyzed as a completely randomized design using the repeated measure analysis of the PROC MIXED procedure of SAS. The results indicated that the inclusion of up to 30 and 9.0% HS and HO, respectively, to diets of laying hens had no significant effects on hen performance, egg quality, or plasma level of metabolites (proteins, glucose, uric acid, and cholesterol) and electrolytes (Na, K, Cl, P, and Ca). Overall plasma enzyme concentrations, particularly gamma-glutamyl transferase, were significantly (P < 0.01) lowest at the 10 and 20% levels of HS inclusion, or at the 4.5% HO level of inclusion of the hempseed products compared with the higher levels or control fed hens. Similar effects were also observed for plasma aspartate aminotransferase levels but with the HS enriched diets only (P < 0.05), particularly being lowest at the inclusion levels of 10 and 20% HS compared with the control. The results may imply a possible protective effect of HS- and HO-containing diets, particularly at 10% HS, 20% HS, and 4.5% HO levels, on liver damage/injury. In summary, both HO and HS appear to be well tolerated by laying hens as judged by markers of plasma clinical chemistry supporting the safety and efficacy of hemp products for use in laying hen rations.
本研究的目的是比较给产蛋母鸡饲喂含大麻产品的日粮后,母鸡的生产性能(采食量、产蛋率、蛋重和体重增加)、蛋品质及血液生化指标(酶、电解质、蛋白质和其他血浆成分)。48只罗曼LSL-经典品系(白壳蛋鸡;19周龄)被单独关入笼中,饲喂6种基于小麦-大麦-大豆的日粮中的1种,为期12周。日粮包括大麻籽(HS;10%、20%或30%)、大麻籽油(HO;4.5%或9.0%)或对照日粮(玉米油基)。所有日粮均配制为含有相似水平的粗脂肪(11%)、能量(2800千卡/千克)和粗蛋白(17%)。使用SAS的PROC MIXED过程的重复测量分析,将数据作为完全随机设计进行分析。结果表明,在产蛋母鸡日粮中分别添加高达30%的HS和9.0%的HO,对母鸡生产性能、蛋品质或代谢物(蛋白质、葡萄糖、尿酸和胆固醇)及电解质(钠、钾、氯、磷和钙)的血浆水平没有显著影响。与较高添加水平或对照日粮组的母鸡相比,在添加10%和20%HS或4.5%HO水平的大麻籽产品的日粮组中,总体血浆酶浓度,尤其是γ-谷氨酰转移酶浓度显著最低(P<0.01)。血浆天冬氨酸氨基转移酶水平也观察到类似影响,但仅在富含HS的日粮组中(P<0.0),与对照组相比,在添加10%和20%HS水平时尤其最低。结果可能意味着含HS和HO的日粮,尤其是在10%HS、20%HS和4.5%HO水平时,对肝脏损伤可能具有保护作用。总之,从血浆临床化学指标判断,HO和HS似乎都能被产蛋母鸡很好地耐受,这支持了大麻产品用于产蛋鸡日粮的安全性和有效性。