Ovchinnikova Olga G, Shashkov Alexander S, Moryl Magdalena, Liu Bin, Rozalski Antoni, Knirel Yuriy A
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia.
Department of Immunobiology of Bacteria, Institute of Microbiology, Biotechnology and Immunology, University of Lodz, PL 90-237 Lodz, Poland.
Carbohydr Res. 2014 Oct 29;398:72-6. doi: 10.1016/j.carres.2014.07.008. Epub 2014 Jul 21.
O-Polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Providencia alcalifaciens O45:H25 and studied by sugar analysis, Smith degradation, and (1)H and (13)C NMR spectroscopy. The following structure of the pentasaccharide repeat of the O-polysaccharide was established: [structure: see text]. The O-antigen gene cluster of P. alcalifaciens O45 was sequenced and found to be in full agreement with the O-polysaccharide structure established.
通过对产碱普罗威登斯菌O45:H25脂多糖进行温和酸降解获得O-多糖，并通过糖分析、史密斯降解以及¹H和¹³C NMR光谱对其进行了研究。确定了O-多糖五糖重复单元的以下结构：[结构：见正文]。对产碱普罗威登斯菌O45的O抗原基因簇进行了测序，发现与所确定的O-多糖结构完全一致。
