Marín-Béjar Oskar, Huarte Maite
Division of Oncology, Center for Applied Medical Research (CIMA), University of Navarra, Avda. Pío XII 55, 31008, Pamplona, Spain.
Methods Mol Biol. 2015;1206:87-95. doi: 10.1007/978-1-4939-1369-5_8.
Recent advances in genomics have revealed that cells encode thousands of noncoding RNA molecules that do not require translation to proteins to perform their biological roles. Although very little is known about the mechanisms of function of noncoding RNAs, these undoubtedly involve the interaction with multiple cellular proteins. Here we describe a detailed RNA pulldown protocol for the in vitro detection of proteins specifically interacting with a long RNA molecule.
基因组学的最新进展表明，细胞编码数千种非编码RNA分子，这些分子无需翻译成蛋白质即可发挥其生物学作用。尽管对非编码RNA的功能机制了解甚少，但它们无疑涉及与多种细胞蛋白的相互作用。在此，我们描述了一种详细的RNA下拉实验方案，用于体外检测与长RNA分子特异性相互作用的蛋白质。
