Pagani Giulia, Pandini Cecilia, Gandellini Paolo
Department of Biosciences, University of Milan, 20133 Milan, Italy.
Noncoding RNA. 2022 Oct 26;8(6):74. doi: 10.3390/ncrna8060074.
Evidence accumulated over the past decades shows that the number of identified antisense transcripts is continuously increasing, promoting them from transcriptional noise to real genes with specific functions. Indeed, recent studies have begun to unravel the complexity of the antisense RNA (asRNA) world, starting from the multidimensional mechanisms that they can exert in physiological and pathological conditions. In this review, we discuss the multiverse of the molecular functions of asRNAs, describing their action through transcription-dependent and RNA-dependent mechanisms. Then, we report the workflow and methodologies to study and functionally characterize single asRNA candidates.
过去几十年积累的证据表明，已鉴定的反义转录本数量在不断增加，促使它们从转录噪音转变为具有特定功能的真正基因。事实上，最近的研究已经开始揭示反义RNA（asRNA）世界的复杂性，从它们在生理和病理条件下发挥作用的多维机制入手。在这篇综述中，我们讨论了asRNA分子功能的多元性，描述了它们通过转录依赖性和RNA依赖性机制发挥的作用。然后，我们报告了研究单个asRNA候选物并对其进行功能表征的工作流程和方法。
