Department of Pharmaceutical Sciences, School of Life Sciences, North South University, Bashundhara, Dhaka 1229, Bangladesh.
BMC Complement Altern Med. 2014 Sep 22;14:346. doi: 10.1186/1472-6882-14-346.
The present study was aimed to investigate the antinociceptive and anti-inflammatory activity of the Curcuma zedoaria (family Zingiberaceae) ethanolic rhizome extract in laboratory using both in vitro and in vivo methods so as to justify its traditional use in the above mentioned pathological conditions.
Phytochemical screening was done to find the presence of various secondary metabolites of the plant. In vivo antinociceptive activity was performed employing the hot plate method, acidic acid induced writhing test and formalin induced writhing test on Swiss albino mice at doses of 250 and 500 mg/kg body weight. Anti-inflammatory activity test was done on Long Evans rats at two different doses (250 and 500 mg/kg body weight) by using carrageenan induced paw edema test. Finally in vitro anti-inflammatory test by protein-denaturation method was followed. Data were analyzed by one-way analysis of variance (ANOVA) and Dunnett's t-test was used as the test of significance. P value <0.05 was considered as the minimum level of significance.
Phytochemical screening revealed presence of tannins, saponins, flavonoids, gums & carbohydrates, steroids, alkaloids, reducing sugars and terpenoids in the extract. In the hot plate method, the extract increased the reaction time of heat sensation significantly to 61.99% and 78.22% at the doses of 250 and 500 mg/kg BW respectively. In acetic acid induced writhing test, the percent inhibition of writhing response by the extract was 48.28% and 54.02% at 250 and 500 mg/kg doses respectively (p < 0.001). The extract also significantly inhibited the licking response in both the early phase (64.49%, p < 0.01) and the late phase (62.37%, p < 0.01) in formalin induced writhing test. The extract significantly (p < 0.05, p < 0.01 and p < 0.001) inhibited carrageenan induced inflammatory response in rats in a dose related manner. In in-vitro anti-inflammatory test, the extract significantly inhibited protein denaturation of 77.15, 64.43, 53.04, 36.78 and 23.70% for doses of 500, 400, 300, 200 and 100 μg/mL respectively.
The results obtained from the tests indicate that the plant might have one or more secondary metabolite(s) having central and peripheral analgesic and anti-inflammatory activity.
本研究旨在通过体内和体外方法研究姜黄（姜科）根茎的乙醇提取物的抗伤害和抗炎活性，以证明其在上述病理情况下的传统用途。
进行植物化学筛选以确定植物中存在的各种次生代谢物。在 250 和 500 mg/kg 体重的剂量下，通过热板法、酸诱导扭体试验和福尔马林诱导扭体试验对瑞士白化小鼠进行体内镇痛活性试验。在两个不同剂量（250 和 500 mg/kg 体重）下，通过角叉菜胶诱导的足肿胀试验对长耳大鼠进行抗炎活性试验。最后，采用蛋白质变性法进行体外抗炎试验。通过单因素方差分析（ANOVA）对数据进行分析，并使用 Dunnett's t 检验作为显著性检验。p 值<0.05 被认为是最低显著性水平。
植物化学筛选显示提取物中存在单宁、皂苷、类黄酮、树胶和碳水化合物、甾体、生物碱、还原糖和萜类化合物。在热板法中，提取物分别使热感觉的反应时间增加至 61.99%和 78.22%，剂量为 250 和 500 mg/kg BW。在醋酸诱导扭体试验中，提取物对扭体反应的抑制率分别为 48.28%和 54.02%，剂量为 250 和 500 mg/kg。提取物还显著抑制了福尔马林诱导的扭体试验中早期（64.49%，p<0.01）和晚期（62.37%，p<0.01）的舔舐反应。提取物在剂量依赖性方式下显著（p<0.05，p<0.01 和 p<0.001）抑制角叉菜胶诱导的大鼠炎症反应。在体外抗炎试验中，提取物分别以 500、400、300、200 和 100 μg/mL 的剂量显著抑制 77.15%、64.43%、53.04%、36.78%和 23.70%的蛋白质变性。
试验结果表明，该植物可能含有一种或多种具有中枢和外周镇痛和抗炎活性的次生代谢物。
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