Moody J O, Robert V A, Connolly J D, Houghton P J
Department of Pharmacognosy, Faculty of Pharmacy, University of Ibadan, Ibadan, Nigeria.
J Ethnopharmacol. 2006 Mar 8;104(1-2):87-91. doi: 10.1016/j.jep.2005.08.051. Epub 2005 Oct 19.
Sphenocentrum jollyanum crude extracts and an isolated constituent were evaluated for anti-inflammatory activity using the carrageenan-induced hind paw oedema of healthy adult albino rats and utilizing the oral route of administration. The fruit methanol extract (79.58% inhibition at 200 mg kg(-1)) gave a higher anti-inflammatory activity than the root extract (53.75% inhibition at 200 mg ml(-1)). Further purification of the most active fruit methanol extract (MFE) led to the isolation of three furanoditerpenes identified as columbin, isocolumbin, fibleucin (uv, ir, nmr and ms) as well as a flavonoid-rich fraction (FDE). Both columbin (67.08% inhibition at 20 mg kg(-1), p<0.05) and FDE (76.25% inhibition at 200 mg kg(-1); p<0.05) gave significant anti-inflammatory activities in comparable range with reference acetylsalicylic acid (72.5% inhibition at 100 mg kg(-1)). The results provide some justification for the folkloric uses of Sphenocentrum jollyanum in the treatment of inflammatory-based diseases across the West African sub-region.
采用角叉菜胶诱导健康成年白化大鼠后爪水肿模型，并通过口服给药途径，对蝶豆粗提物及其分离成分的抗炎活性进行了评价。果实甲醇提取物（200 mg kg(-1)时抑制率为79.58%）比根提取物（200 mg ml(-1)时抑制率为53.75%）具有更高的抗炎活性。对活性最高的果实甲醇提取物（MFE）进一步纯化，得到了三种呋喃二萜，分别鉴定为哥伦宾、异哥伦宾、费布勒辛（通过紫外、红外、核磁共振和质谱鉴定）以及富含黄酮的组分（FDE）。哥伦宾（20 mg kg(-1)时抑制率为67.08%，p<0.05）和FDE（200 mg kg(-1)时抑制率为76.25%；p<0.05）均表现出显著的抗炎活性，与参比乙酰水杨酸（100 mg kg(-1)时抑制率为72.5%）相当。这些结果为蝶豆在西非次区域用于治疗炎症性疾病的民间用途提供了一定的依据。
