Subhash Y, Sasikala Ch, Ramana Ch V
Department of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad 500 046, India.
Bacterial Discovery Laboratory, Centre for Environment, Institute of Science and Technology, J. N. T. University, Kukatpally, Hyderabad 500 085, India.
Int J Syst Evol Microbiol. 2014 Dec;64(Pt 12):4129-4133. doi: 10.1099/ijs.0.066225-0. Epub 2014 Sep 21.
Strain JC245(T) was isolated from a sand sample, and appeared as dark pink colonies on agar plates with cells staining Gram-negative. Catalase and oxidase activities were positive. Casein was hydrolysed while chitin, gelatin and starch were not. Major (>5 %) fatty acids were iso-C15 : 0, iso-C15 : 0 3-OH, C16 : 1ω5c, C16 : 1ω6c/C16 : 1ω7c, anteiso-C17 : 1 B/iso-C17 : 1 I and iso-C17 : 0 3-OH. Strain JC245(T) contained phosphatidylethanolamine and two unidentified lipids as the major polar lipids, with minor amounts of four unidentified lipids and an unidentified amino lipid. Bacterial hopane derivatives and adenosylhopane were the major hopanoids. Hydroxyflexixanthin was identified as one of the major carotenoids of strain JC245(T) along with five unidentified carotenoids. The genomic DNA G+C content was 52.5 mol%. 16S rRNA gene sequence comparisons indicated that strain JC245(T) represents a member of the genus Hymenobacter within the family Cytophagaceae of the phylum Bacteroidetes. Strain JC245(T) shared the highest 16S rRNA gene sequence similarity with Hymenobacter roseosalivarius AA-718(T) (98.3 %) and other members of the genus Hymenobacter (<95.1 %). However, strain JC245(T) showed 21±2 % relatedness (based on DNA-DNA hybridization) with H. roseosalivarius DSM 11622(T). Distinct morphological, physiological and genotypic differences from previously described taxa support the classification of strain JC245(T) as a representative of a novel species in the genus Hymenobacter, for which the name Hymenobacter roseus sp. nov. is proposed. The type strain is JC245(T) ( = KCTC 42090(T) = LMG 28260(T)).
菌株JC245(T)从一份沙子样本中分离得到，在琼脂平板上呈现深粉色菌落，细胞革兰氏染色阴性。过氧化氢酶和氧化酶活性呈阳性。能水解酪蛋白，而几丁质、明胶和淀粉则不能被水解。主要（>5%）脂肪酸为异-C15 : 0、异-C15 : 0 3-OH、C16 : 1ω5c、C16 : 1ω6c/C16 : 1ω7c、anteiso-C17 : 1 B/异-C17 : 1 I和异-C17 : 0 3-OH。菌株JC245(T)含有磷脂酰乙醇胺和两种未鉴定的脂质作为主要极性脂质，还有少量四种未鉴定的脂质和一种未鉴定的氨基脂质。细菌藿烷衍生物和腺苷藿烷是主要的藿烷类化合物。羟基柔膜菌黄素与五种未鉴定的类胡萝卜素一起被鉴定为菌株JC245(T)的主要类胡萝卜素之一。基因组DNA的G+C含量为52.5 mol%。16S rRNA基因序列比较表明，菌株JC245(T)代表拟杆菌门噬细胞科海杆菌属的一个成员。菌株JC245(T)与玫瑰唾液海杆菌AA-718(T)的16S rRNA基因序列相似性最高（98.3%），与海杆菌属的其他成员相似性小于95.1%。然而，菌株JC245(T)与玫瑰唾液海杆菌DSM 11622(T)的相关性为21±2%（基于DNA-DNA杂交）。与先前描述的分类单元在形态、生理和基因型上的明显差异支持将菌株JC245(T)分类为海杆菌属一个新物种的代表，为此提出新物种名称玫瑰海杆菌（Hymenobacter roseus sp. nov.）。模式菌株为JC245(T)（=KCTC 42090(T)=LMG 28260(T)）。
