Department of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad 500 046, India.
Bacterial Discovery Laboratory, Centre for Environment, Institute of Science and Technology, J. N. T. University, Kukatpally, Hyderabad 500 085, India.
Int J Syst Evol Microbiol. 2014 Jul;64(Pt 7):2238-2243. doi: 10.1099/ijs.0.061937-0. Epub 2014 Apr 7.
Strain JC216(T) was isolated from a contaminated Petri dish. Colonies were of pale yellow colour and cells were Gram-stain-negative, oxidase-positive and catalase-positive. Chitin, starch and gelatin were not hydrolysed. Strain JC216(T) contained C18 : 1ω7c/C18 : 1ω6c, C16 : 1ω7c/C16 1ω6c, C14 : 0 2-OH and C16 : 0 as the major (≥ 8%) fatty acids with minor amounts of C12 : 0, C15 : 0 2-OH, C16 : 0 2-OH, C16 : 1 2-OH, C17 : 1ω6c, C17 : 1ω8c and C17 : 1ω9c. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and sphingoglycolipid were the major polar lipids. Minor amounts of unidentified amino lipids and unidentified lipids were also detected. The major hopanoids identified were bacterial hopane derivatives and diplopterol. Minor amounts of diploptene and an unidentified hopanoid were also present. Spermidine was the major polyamine with minor amounts of sym-homospermidine and putrescine. N-Acetylglucosamine and fructose were identified as major cell-wall sugars along with minor amounts of mannose and galactose. The genomic DNA G+C content was 55 mol%. Comparisons of the 16S rRNA gene sequence indicated that strain JC216(T) represents a member of the genus Sphingopyxis in the family Sphingomonadaceae within the class Alphaproteobacteria. Strain JC216(T) had a sequence similarity of 97.28% with Sphingopyxis wooponensis 03SU3-P(T) and <96.71% with other members of the family Sphingomonadaceae. Furthermore, strain JC216(T) had 33 ± 1% relatedness (based on DNA-DNA hybridization) with S. wooponensis KCTC 23340(T) ( = 03SU3-P(T)). Distinct morphological, physiological and genotypic differences from the previously described taxa support the classification of strain JC216(T) as a representative of a novel species in the genus Sphingopyxis, for which the name Sphingopyxis contaminans sp. nov. is proposed. The type strain is JC216(T) ( = KCTC 32445(T) = LMG 27671(T)).
JC216(T) 菌株从污染的培养皿中分离得到。菌落呈浅黄色，细胞革兰氏染色阴性，氧化酶阳性，接触酶阳性。几丁质、淀粉和明胶不被水解。JC216(T) 菌株含有 C18 : 1ω7c/C18 : 1ω6c、C16 : 1ω7c/C16 1ω6c、C14 : 0 2-OH 和 C16 : 0 作为主要（≥8%）脂肪酸，少量 C12 : 0、C15 : 0 2-OH、C16 : 0 2-OH、C16 1-OH、C16 : 1 2-OH、C17 : 1ω6c、C17 : 1ω8c 和 C17 : 1ω9c。双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱和鞘糖脂是主要的极性脂。还检测到少量未鉴定的氨基酸脂和未鉴定的脂。鉴定出的主要藿烷类化合物为细菌藿烷衍生物和双孢菌素。还存在少量双孢菌素和一种未鉴定的藿烷。亚精胺是主要的多胺，其次是 sym-homospermidine 和腐胺。N-乙酰葡萄糖胺和果糖是主要的细胞壁糖，其次是少量的甘露糖和半乳糖。基因组 DNA G+C 含量为 55mol%。16S rRNA 基因序列比较表明，JC216(T) 株代表鞘氨醇单胞菌科鞘氨醇属中的一个成员，属于α变形菌纲。JC216(T) 株与 Sphingopyxis wooponensis 03SU3-P(T) 的 16S rRNA 基因序列相似性为 97.28%，与鞘氨醇单胞菌科其他成员的相似性<96.71%。此外，JC216(T) 株与 Sphingopyxis wooponensis KCTC 23340(T)（=03SU3-P(T)）的 DNA-DNA 杂交同源性为 33±1%。与先前描述的分类群相比，JC216(T) 株在形态、生理和基因型上存在明显差异，支持将其分类为鞘氨醇属的一个新种，命名为 Sphingopyxis contaminans sp. nov.。模式株为 JC216(T)（=KCTC 32445(T)=LMG 27671(T)）。
