Department of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad 500046, India.
Bacterial Discovery Laboratory, Centre for Environment, Institute of Science and Technology, J.N.T. University Hyderabad, Kukatpally, Hyderabad 500085, India.
Int J Syst Evol Microbiol. 2013 Dec;63(Pt 12):4524-4532. doi: 10.1099/ijs.0.052183-0. Epub 2013 Aug 1.
Two bacterial strains (JC130(T) and JA747(T)) were isolated from dry soils of a solar saltern. Phylogenetic analysis showed that strain JA747(T) clustered with species of the genus Erythrobacter belonging to the family Erythrobacteraceae of the class Alphaproteobacteria in the phylum Proteobacteria, while strain JC130(T) clustered with species of the genus Pontibacter belonging to the family Cytophagaceae of the phylum Bacteroidetes. Based on 16S rRNA gene sequence analysis, strain JA747(T) had highest similarity with Erythrobacter gangjinensis K7-2(T) (96.7 %) and other members of the genus Erythrobacter (<96 %). Strain JC130(T) had highest sequence similarity with Pontibacter korlensis X14-1(T) (98.1 %), Pontibacter actiniarum KMM 6156(T) (96.9 %) and other members of the genus Pontibacter (<96 %). However, strain JC130(T) showed less than 32 % DNA reassociation value (based on DNA-DNA hybridization) with Pontibacter korlensis NRRL B-51097(T) ( = X14-1(T)) and Pontibacter actiniarum LMG 23027(T) ( = KMM 6156(T)). Strain JA747(T) was positive for catalase and oxidase activity and negative for nitrate reduction, and hydrolysis of starch and casein. Phosphatidylglycerol, diphostatidylglycerol, phosphatidylethanolamine and phosphatidylcholine were the major polar lipids for strain JA747(T). C18 : 1ω7c, C16 : 1ω6c/C16 : 1ω7c, C17 : 1ω6c and C16 : 0 were the major fatty acids of strain JA747(T). Strain JC130(T) was positive for catalase and oxidase, and negative for gelatinase and nitrate reduction. Phosphatidylethanolamine was the major polar lipid of strain JC130(T). Major fatty acids of strain JC130(T) were iso-C15 : 0 and summed feature 4 (anteiso-C17 : 1B/iso I). Based on the phenotypic, chemotaxonomic and molecular evidence presented, strains JA747(T) and JC130(T) are considered to represent two novel species of the genera Erythrobacter and Pontibacter, for which the names Erythrobacter odishensis sp. nov. (type strain JA747(T) = KCTC 23981(T) = NBRC 108930(T)) and Pontibacter odishensis sp. nov. (type strain JC130(T) = KCTC 23982(T) = LMG 26962(T)), respectively, are proposed. Emended descriptions of the genera Erythrobacter and Pontibacter are provided.
两株细菌（JC130(T)和 JA747(T)）从太阳能盐场的干燥土壤中分离得到。系统发育分析表明，JA747(T)菌株与红杆菌属的物种聚在一起，属于变形菌门的 α-变形菌纲红杆菌科，而 JC130(T)菌株与粘球菌科的 Pontibacter 属的物种聚在一起。基于 16S rRNA 基因序列分析，JA747(T)菌株与金氏红杆菌 K7-2(T)（96.7%）和其他红杆菌属的物种具有最高相似性（<96%）。JC130(T)菌株与科拉李斯特氏菌 X14-1(T)（98.1%）、活性粘球菌 KMM 6156(T)（96.9%）和其他 Pontibacter 属的物种具有最高序列相似性（<96%）。然而，JC130(T)菌株与科拉李斯特氏菌 NRRL B-51097(T)（=X14-1(T)）和活性粘球菌 LMG 23027(T)（=KMM 6156(T)）的 DNA 同源性小于 32%（基于 DNA-DNA 杂交）。JA747(T)菌株过氧化氢酶和氧化酶活性阳性，硝酸盐还原、淀粉水解和酪蛋白水解阴性。磷脂酰甘油、双磷脂酰甘油、磷脂酰乙醇胺和磷脂酰胆碱是 JA747(T)菌株的主要极性脂。C18:1ω7c、C16:1ω6c/C16:1ω7c、C17:1ω6c 和 C16:0 是 JA747(T)菌株的主要脂肪酸。JC130(T)菌株过氧化氢酶和氧化酶阳性，明胶酶和硝酸盐还原阴性。磷脂酰乙醇胺是 JC130(T)菌株的主要极性脂。JC130(T)菌株的主要脂肪酸为 iso-C15:0 和总和特征 4（anteiso-C17:1B/iso I）。基于表型、化学分类和分子证据，菌株 JA747(T)和 JC130(T)被认为代表红杆菌属和 Pontibacter 属的两个新种，分别命名为 Odishan 红杆菌（Erythrobacter odishensis sp. nov.，模式菌株 JA747(T)=KCTC 23981(T)=NBRC 108930(T)）和 Odishan 粘球菌（Pontibacter odishensis sp. nov.，模式菌株 JC130(T)=KCTC 23982(T)=LMG 26962(T)）。对红杆菌属和 Pontibacter 属的描述进行了修正。
