Wang Baichuan, Sun Caixia, Shao Zengwu, Yang Shuhua, Che Biao, Wu Qiang, Liu Jianxiang
Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Department of Gynecology, General Hospital of the Yangtze River Shipping, Wuhan 430022, China.
Biomed Res Int. 2014;2014:421954. doi: 10.1155/2014/421954. Epub 2014 Aug 26.
Designer self-assembling peptide nanofiber hydrogel scaffolds have been considered as promising biomaterials for tissue engineering because of their excellent biocompatibility and biofunctionality. Our previous studies have shown that a novel designer functionalized self-assembling peptide nanofiber hydrogel scaffold (RLN/RADA16, LN-NS) containing N-terminal peptide sequence of link protein (link N) can promote nucleus pulposus cells (NPCs) adhesion and three-dimensional (3D) migration and stimulate biosynthesis of type II collagen and aggrecan by NPCs in vitro. The present study has extended these investigations to determine the effects of this functionalized LN-NS on bone marrow stem cells (BMSCs), a potential cell source for NP regeneration. Although the functionalized LN-NS cannot promote BMSCs proliferation, it significantly promotes BMSCs adhesion compared with that of the pure RADA16 hydrogel scaffold. Moreover, the functionalized LN-NS remarkably stimulates biosynthesis and deposition of type II collagen and aggrecan. These data demonstrate that the functionalized peptide nanofiber hydrogel scaffold containing link N peptide as a potential matrix substrate will be very useful in the NP tissue regeneration.
由于具有出色的生物相容性和生物功能，设计型自组装肽纳米纤维水凝胶支架被认为是用于组织工程的有前景的生物材料。我们之前的研究表明，一种新型的设计型功能化自组装肽纳米纤维水凝胶支架（RLN/RADA16，LN-NS），其包含连接蛋白的N端肽序列（连接N），在体外可促进髓核细胞（NPCs）的黏附、三维（3D）迁移，并刺激NPCs合成II型胶原蛋白和聚集蛋白聚糖。本研究进一步拓展这些研究，以确定这种功能化的LN-NS对骨髓干细胞（BMSCs）的影响，BMSCs是NP再生的一种潜在细胞来源。尽管功能化的LN-NS不能促进BMSCs增殖，但与纯RADA16水凝胶支架相比，它能显著促进BMSCs的黏附。此外，功能化的LN-NS能显著刺激II型胶原蛋白和聚集蛋白聚糖的生物合成与沉积。这些数据表明，含有连接N肽的功能化肽纳米纤维水凝胶支架作为一种潜在的基质底物，在NP组织再生中将非常有用。
