Institute of Neurophysiology, Medical Faculty, University of Cologne , Cologne , Germany ; Clinic of Neurosurgery, Medical Faculty, University of Cologne , Cologne , Germany.
Institute of Neurophysiology, Medical Faculty, University of Cologne , Cologne , Germany.
Front Cell Neurosci. 2014 Sep 5;8:257. doi: 10.3389/fncel.2014.00257. eCollection 2014.
Stem cells have been demonstrated to possess a therapeutic potential in experimental models of various central nervous system disorders, including stroke. The types of implanted cells appear to play a crucial role. Previously, groups of the stem cell network NRW implemented a feeder-based cell line within the scope of their projects, examining the implantation of stem cells after ischemic stroke and traumatic brain injury. Retrospective evaluation indicated the presence of spindle-shaped cells in several grafts implanted in injured animals, which indicated potential contamination by co-cultured feeder cells (murine embryonic fibroblasts - MEFs). Because feeder-based cell lines have been previously exposed to a justified criticism with regard to contamination by animal glycans, we aimed to evaluate the effects of stem cell/MEF co-transplantation. MEFs accounted for 5.3 ± 2.8% of all cells in the primary FACS-evaluated co-culture. Depending on the culture conditions and subsequent purification procedure, the MEF-fraction ranged from 0.9 to 9.9% of the cell suspensions in vitro. MEF survival and related formation of extracellular substances in vivo were observed after implantation into the uninjured rat brain. Impurity of the stem cell graft by MEFs interferes with translational strategies, which represents a threat to the potential recipient and may affect the graft microenvironment. The implications of these findings are critically discussed.
干细胞在多种中枢神经系统疾病（包括中风）的实验模型中表现出治疗潜力。植入细胞的类型似乎起着至关重要的作用。此前，NRW 干细胞网络的研究小组在其项目范围内实施了基于饲养细胞的细胞系，研究了缺血性中风和创伤性脑损伤后的干细胞植入。回顾性评估表明，在受伤动物植入的几个移植物中存在梭形细胞，这表明可能被共培养的饲养细胞（小鼠胚胎成纤维细胞-MEFs）污染。由于基于饲养细胞的细胞系以前曾因动物糖胺聚糖的污染而受到合理的批评，我们旨在评估干细胞/MEF 共移植的效果。MEF 占原代 FACS 评估共培养物中所有细胞的 5.3±2.8%。根据培养条件和随后的纯化程序，MEF 分数在体外细胞悬液中从 0.9%到 9.9%不等。在植入未受伤大鼠脑后，观察到 MEF 的存活及其相关的细胞外物质的形成。MEF 对干细胞移植物的污染会干扰转化策略，这对潜在受体构成威胁，并可能影响移植物微环境。这些发现的意义受到了批判性的讨论。
