Taghizadeh-Kermani Ali, Jafarian Amir Hossein, Ashabyamin Reza, Seilanian-Toosi Mehdi, Pourali Leila, Asadi Mehdi, Mashhadi Leila
Surgical Oncology Research Center, Imam Reza Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran ; Cancer Research Center, Omid Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Cancer Molecular Pathology Research Center, Ghaem Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Iran J Cancer Prev. 2014 Winter;7(1):17-21.
Breast carcinoma is the most common non-skin malignancy in women. More recently, it has been suggested that extracellular proteinase has also regulated growth factors and cytokines that might contribute to tumor progression. CD10 is a 90-110kd cell surface zinc-dependent metalloproteinase. Since CD10 is structurally similar to matrix metalloproteinase and stromelysin, it might facilitate cancer cell invasion and/or metastasis. The aim of this study was investigation the rate of CD10 expression in the stromal cells of invasive ductal breast carcinomas, Immunohistochemical aspects, then any other aspects to be able to clarify its correlation with other clinicopathological factors of this disease.
One hundred patients with histopathologic diagnosis of invasive ductal carcinoma and 50 patients with fibroadenoma of breast (as the control group) have selected, then 150 paraffin blocks have obtained. The stained slides by immunohistochemistry method for CD10 marker have examined separately by two pathologists, and discrepancies have reviewed in common session to get the final result.
Stromal CD10 has detected in 28% of the IDC. No kind of immunoreactivity has identified in the stromal cells of normal breast. Stromal CD10 expression in IDC has significantly correlated with increasing tumor size (p<0.001), increasing histologic grade (p<0.001), the presence of nodal metastases (p<0.001) and estrogen receptor negative status (p=0.003).
Stromal CD10 expression in IDC has closely correlated with invasion and metastasis and it might play an important role in the pathogenesis of IDC.
乳腺癌是女性最常见的非皮肤恶性肿瘤。最近，有人提出细胞外蛋白酶也调节可能有助于肿瘤进展的生长因子和细胞因子。CD10是一种90 - 110kd的细胞表面锌依赖性金属蛋白酶。由于CD10在结构上与基质金属蛋白酶和基质溶解素相似，它可能促进癌细胞的侵袭和/或转移。本研究的目的是调查浸润性导管癌基质细胞中CD10的表达率、免疫组化情况，以及其他任何能够阐明其与该疾病其他临床病理因素相关性的方面。
选取100例经组织病理学诊断为浸润性导管癌的患者和50例乳腺纤维瘤患者（作为对照组），共获得150个石蜡块。由两位病理学家分别检查免疫组化方法检测CD10标记的染色切片，共同讨论差异以得出最终结果。
在28%的浸润性导管癌中检测到基质CD10。在正常乳腺的基质细胞中未发现任何免疫反应性。浸润性导管癌中基质CD10的表达与肿瘤大小增加（p<0.001）、组织学分级增加（p<0.001）、淋巴结转移的存在（p<0.001）和雌激素受体阴性状态（p = 0.003）显著相关。
浸润性导管癌中基质CD10的表达与侵袭和转移密切相关，可能在浸润性导管癌的发病机制中起重要作用。
