Prylutskyy Yu I, Evstigneev M P, Pashkova I S, Wyrzykowski D, Woziwodzka A, Gołuński G, Piosik J, Cherepanov V V, Ritter U
Taras Shevchenko National University of Kyiv, Volodymyrska Str. 64, 01601 Kyiv, Ukraine.
Phys Chem Chem Phys. 2014 Nov 14;16(42):23164-72. doi: 10.1039/c4cp03367a.
The aim of this paper was to provide the physico-chemical characterization of a key process leading to amplification of the antitumor effect of antibiotic Doxorubicin (Dox) in vivo and in vitro and occurring at the molecular level through complexation with C60 fullerene. A wide range of physico-chemical tools was used such as UV/Vis and NMR spectroscopies, atomic force microscopy, isothermal titration calorimetry and zeta-potential methods. The unusual thermodynamic behavior of the complexation process was reported, featuring unexpected and, to a certain extent, contradictory experimental observations. The explanation of the obtained results was proposed resulting in creation of a general view on aromatic drug binding with C60 fullerene. Based on these results some important practical outcomes for anticancer therapy were formulated.
本文的目的是提供一个关键过程的物理化学特性，该过程导致抗生素阿霉素（Dox）在体内和体外抗肿瘤作用的放大，并通过与C60富勒烯络合在分子水平上发生。使用了广泛的物理化学工具，如紫外/可见光谱和核磁共振光谱、原子力显微镜、等温滴定量热法和zeta电位法。报道了络合过程异常的热力学行为，其具有意想不到的且在一定程度上相互矛盾的实验观察结果。对所得结果进行了解释，从而形成了关于芳香族药物与C60富勒烯结合的总体观点。基于这些结果，制定了一些抗癌治疗的重要实际成果。
