Borowik Agnieszka, Butowska Kamila, Konkel Kinga, Banasiuk Rafał, Derewonko Natalia, Wyrzykowski Dariusz, Davydenko Mykola, Cherepanov Vsevolod, Styopkin Viktor, Prylutskyy Yuriy, Pohl Paweł, Krolicka Aleksandra, Piosik Jacek
Laboratory of Biophysics, Intercollegiate Faculty of Biotechnology UG-MUG, University of Gdansk, 80-307 Gdansk, Poland.
Laboratory of Biologically Active Compounds, IFB UG-MUG, University of Gdansk, 80-307 Gdansk, Poland.
Nanomaterials (Basel). 2019 Jul 3;9(7):973. doi: 10.3390/nano9070973.
Among metal-based nanoparticles, silver nanoparticles (AgNPs) are particularly appealing because of their stability, functionality, and documented antimicrobial properties. AgNPs also offer the possibility of different surface modifications. In this work, we functionalized AgNPs with thiobarbituric acid or 11-mercaptoundecanoic acid residues to improve the nanoparticles' biological activities. Subsequently, we assessed the physicochemical properties of newly synthesized AgNPs using a wide range of biophysical methodologies, including UV/vis and fluorescence spectroscopy, atomic force and scanning electron microscopy, and dynamic light scattering and isothermal titration calorimetry. Next, we examined the effect of nanoparticles functionalization on AgNPs mutagenicity and toxicity. Our study revealed that AgNPs' surface modification affects nanoparticles aggregation, and also impacts nanoparticles' interaction with model acridine mutagen ICR-191. AgNPs coated with MUA showed the most interesting interactions with tested ICR-191, slightly modulating its toxicity properties by decreasing the viability in treated cells.
在金属基纳米颗粒中，银纳米颗粒（AgNPs）因其稳定性、功能性和已记录的抗菌特性而格外引人注目。AgNPs还提供了不同表面修饰的可能性。在这项工作中，我们用硫代巴比妥酸或11-巯基十一烷酸残基对AgNPs进行功能化，以提高纳米颗粒的生物活性。随后，我们使用多种生物物理方法评估了新合成的AgNPs的物理化学性质，包括紫外/可见光谱和荧光光谱、原子力显微镜和扫描电子显微镜，以及动态光散射和等温滴定量热法。接下来，我们研究了纳米颗粒功能化对AgNPs致突变性和毒性的影响。我们的研究表明，AgNPs的表面修饰会影响纳米颗粒的聚集，也会影响纳米颗粒与模型吖啶诱变剂ICR-191的相互作用。涂有MUA的AgNPs与测试的ICR-191表现出最有趣的相互作用，通过降低处理细胞的活力来轻微调节其毒性特性。
