Chaim Felipe Mendonça, Sato Daniela Tiemi, Rodrigues Murilo Rocha, Dias Alice Moreira, Silveira Júnior Paulo Pedroso, Pereira José Aires, Martinez Carlos Augusto Real
Sao Francisco University, Bragança Paulista, SP, Brazil.
School of Medicine, USF, Bragança Paulista, SP, Brazil.
Acta Cir Bras. 2014 Sep;29(9):544-52. doi: 10.1590/s0102-8650201400150001.
To evaluate the effects of sucralfate on tissue content of neutral and acids mucins in rats with diversion colitis.
Thirty-six rats were submitted to a proximal right colostomy and a distal mucous fistula. They were divided into two groups according to sacrifice to be performed two or four weeks after intervention. Each group was divided into three subgroups according daily application of enemas containing saline, sucralfate at 1.0 g/kg/day or 2.0 g/kg/day. Colitis was diagnosed by histological analysis and neutral and acid mucins by Periodic Acid Schiff and Alcian Blue techniques, respectively. The contents of mucins were quantified by computer-assisted image analysis. Student's t paired and ANOVA test were used to compare the contents of both types of mucins among groups, and to verify the variance with time, establishing level of signification of 5% for both (p<0.05).
Enemas containing sucralfate improves the inflammation and increases the tissue contents of neutral and acid mucins. The content of neutral mucins does not change with the time or concentration of sucralfate used, while acid mucins increases with concentration and time of intervention.
Sucralfate enemas improve the inflammatory process and increase the tissue content of neutral and acid mucins in colon without fecal stream.
评估硫糖铝对转流性结肠炎大鼠中性和酸性黏蛋白组织含量的影响。
36只大鼠接受近端右半结肠造口术和远端黏液瘘手术。根据干预后2周或4周处死大鼠分为两组。每组再根据每日灌肠给予生理盐水、1.0 g/kg/天或2.0 g/kg/天硫糖铝分为三个亚组。通过组织学分析诊断结肠炎，分别用高碘酸希夫氏反应和阿尔辛蓝技术检测中性和酸性黏蛋白。通过计算机辅助图像分析对黏蛋白含量进行定量。采用配对t检验和方差分析比较各组两种黏蛋白的含量，并验证其随时间的变化差异，设定两者的显著性水平均为5%（p<0.05）。
含硫糖铝的灌肠剂可改善炎症，并增加中性和酸性黏蛋白的组织含量。中性黏蛋白的含量不随硫糖铝使用时间或浓度的变化而改变，而酸性黏蛋白的含量随干预浓度和时间增加。
硫糖铝灌肠剂可改善无粪便流的结肠炎症过程，并增加中性和酸性黏蛋白的组织含量。
