IEEE Trans Biomed Circuits Syst. 2015 Jun;9(3):334-44. doi: 10.1109/TBCAS.2014.2346402. Epub 2014 Sep 19.
Lipid bilayer membrane (BLM) arrays are required for high throughput analysis, for example drug screening or advanced DNA sequencing. Complex microfluidic devices are being developed but these are restricted in terms of array size and structure or have integrated electronic sensing with limited noise performance. We present a compact and scalable multichannel electrophysiology platform based on a hybrid approach that combines integrated state-of-the-art microelectronics with low-cost disposable fluidics providing a platform for high-quality parallel single ion channel recording. Specifically, we have developed a new integrated circuit amplifier based on a novel noise cancellation scheme that eliminates flicker noise derived from devices under test and amplifiers. The system is demonstrated through the simultaneous recording of ion channel activity from eight bilayer membranes. The platform is scalable and could be extended to much larger array sizes, limited only by electronic data decimation and communication capabilities.
脂质双层膜（BLM）阵列对于高通量分析是必需的，例如药物筛选或高级 DNA 测序。正在开发复杂的微流控设备，但这些设备在阵列大小和结构方面受到限制，或者具有集成的电子传感功能，噪声性能有限。我们提出了一种基于混合方法的紧凑且可扩展的多通道电生理学平台，该方法结合了最先进的微电子技术和低成本一次性流体技术，为高质量并行单离子通道记录提供了平台。具体来说，我们开发了一种基于新型噪声消除方案的新型集成电路放大器，该方案消除了源自测试器件和放大器的闪烁噪声。该系统通过同时记录八个双层膜中的离子通道活性来进行演示。该平台是可扩展的，可以扩展到更大的阵列尺寸，仅受电子数据抽取和通信能力的限制。
