Ren Caifang, Sun Hongyan, Wang Lizhong, Zhang Guomin, Fan Yixuan, Yan Guangyao, Wang Dan, Wang Feng
Jiangsu Livestock Embryo Engineering Laboratory, Nanjing Agricultural University, Nanjing 210095, China.
Yi Chuan. 2014 Sep;36(9):879-87. doi: 10.3724/SP.J.1005.2014.0879.
Induced pluripotent stem cells (iPSCs) were reprogrammed from somatic cells using specific transcription factors. Bypassing the ethical issue caused by embryonic stem cells (ESCs), iPSCs can be successfully induced from a variety of cells, which makes iPSCs a powerful research tool for developmental biology. iPSCs have also become indispensable to the research of life science due to their broad potential applications. However, it's a big challenge to obtain iPSCs with high quality and genetic stability. Here, we review the research progress of increasing the reprogramming mechanism and genetic stability of iPSCs in order to provide references of reprogramming efficiency of iPSCs, reducing the cost, and addressing key points of iPSCs quality control, further promoting clinical application of the iPSCs.
诱导多能干细胞（iPSC）是利用特定转录因子对体细胞进行重编程而获得的。绕过了胚胎干细胞（ESC）引发的伦理问题，iPSC可成功地从多种细胞中诱导产生，这使得iPSC成为发育生物学研究的有力工具。由于其广泛的潜在应用，iPSC在生命科学研究中也变得不可或缺。然而，获得高质量和遗传稳定性的iPSC是一项巨大的挑战。在此，我们综述了提高iPSC重编程机制和遗传稳定性的研究进展，以便为提高iPSC重编程效率、降低成本以及解决iPSC质量控制要点提供参考，进一步推动iPSC的临床应用。
