Fujikawa Hiroshi, Sakha Mohammad Z
Laboratory of Veterinary Public Health, Faculty of Agriculture Tokyo University of Agriculture and Technology.
Biocontrol Sci. 2014;19(3):121-7. doi: 10.4265/bio.19.121.
A novel competition model developed with the new logistic model and the Lotka-Volterra model successfully predicted the growth of bacteria in mixed culture using the mesophiles Staphylococcus aureus, Escherichia coli, and Salmonella at a constant temperature in our previous studies. In this study, we further studied the prediction of the growth of those bacteria in mixed culture at dynamic temperatures with various initial populations with the competition model. First, we studied the growth kinetics of the species in a monoculture at various constant temperatures ranging from 16℃ to 32℃. With the analyzed data in the monoculture, we then examined the prediction of bacterial growth in mixed culture with two and three species. The growth of the bacteria in the mixed culture at dynamic temperatures was successfully predicted with the model. The residuals between the observed and predicted populations at the data points were <0.5 log at most points, being 83.3% and 84.2% for the two-species mixture and the three-species mixture, respectively. The present study showed that the model could be applied to the competitive growth in mixed culture at dynamic temperature patterns.
在我们之前的研究中，一个基于新逻辑模型和洛特卡 - 沃尔泰拉模型开发的新型竞争模型，成功预测了嗜温菌金黄色葡萄球菌、大肠杆菌和沙门氏菌在恒温下混合培养时细菌的生长情况。在本研究中，我们使用该竞争模型进一步研究了在动态温度下、具有不同初始种群数量时这些细菌在混合培养中的生长预测。首先，我们研究了这些菌种在16℃至32℃不同恒温下的纯培养生长动力学。利用纯培养中的分析数据，我们随后检验了该模型对两种和三种菌种混合培养中细菌生长的预测能力。该模型成功预测了动态温度下混合培养中细菌的生长情况。在大多数数据点上，观察到的种群数量与预测的种群数量之间的残差最多为<0.5 log，两种菌种混合物和三种菌种混合物分别为83.3%和84.2%。本研究表明，该模型可应用于动态温度模式下混合培养中的竞争生长。
