Steinwender Bernd, Thrimawithana Amali H, Crowhurst Ross N, Newcomb Richard D
The New Zealand Institute for Plant & Food Research Limited, Private Bag 92169, Auckland, 1142, New Zealand.
J Mol Evol. 2015 Jan;80(1):42-56. doi: 10.1007/s00239-014-9650-z. Epub 2014 Sep 25.
How new mate recognition systems evolve when changes are required in both the male and female components remains a conundrum. Here, we investigated the molecular basis of pheromone reception in two species of tortricid (leafroller) moth, Ctenopseustis obliquana and C. herana. Male C. obliquana are attracted to a 90:10 blend of (Z)-8-tetradecenyl acetate (Z8-14:OAc) and (Z)-5-tetradecenyl acetate (Z5-14:OAc), whereas C. herana males are attracted to Z5-14:OAc alone. We used a transcriptome sequencing approach from adult male and female antennae to identify 47 olfactory receptors (ORs) from each species and assessed their expression levels in male and female antennae using RNA-Seq counting and quantitative RT-PCR. Three male-biased and one female-biased OR were identified in C. obliquana by quantitative RT-PCR, and four male-biased and one female-biased receptor in C. herana. The male-biased receptors, CoblOR7, CoblOR30, CherOR7, CherOR30, CherOR1a and CherOR1b were tested for their ability to respond to sex pheromone components in a HEK293 cell calcium assay. CoblOR7 and CherOR7 responded to Z8-14:OAc, however, no receptor for Z5-14:OAc was identified. In addition to Z8-14:OAc, CherOR7 also responded to Z7-14:OAc, indicating that this receptor may be under relaxed constraint. Of the 29 amino acid differences between CoblOR7 and CherOR7, significantly more are located in the third and the sixth transmembrane domain regions. Overall, these findings are consistent with studies revealing the presence of neurons tuned to both Z8-14:OAc and Z5-14:OAc in both species, but that for C. herana males, the ability to detect Z8-14:OAc is currently not required.
当雄性和雌性组成部分都需要发生变化时，新的配偶识别系统如何进化仍然是一个难题。在这里，我们研究了两种卷叶蛾（卷叶蛾科），即斜纹卷叶蛾（Ctenopseustis obliquana）和赫氏卷叶蛾（C. herana）信息素接收的分子基础。斜纹卷叶蛾雄性被90:10的（Z）-8-十四碳烯乙酸酯（Z8-14:OAc）和（Z）-5-十四碳烯乙酸酯（Z5-14:OAc）混合物所吸引，而赫氏卷叶蛾雄性仅被Z5-14:OAc所吸引。我们采用转录组测序方法，从成年雄性和雌性触角中鉴定出每个物种的47种嗅觉受体（ORs），并使用RNA-Seq计数和定量RT-PCR评估它们在雄性和雌性触角中的表达水平。通过定量RT-PCR在斜纹卷叶蛾中鉴定出3种雄性偏向和1种雌性偏向的OR，在赫氏卷叶蛾中鉴定出4种雄性偏向和1种雌性偏向的受体。在HEK293细胞钙测定中测试了雄性偏向受体CoblOR7、CoblOR30、CherOR7、CherOR30、CherOR1a和CherOR1b对性信息素成分的反应能力。CoblOR7和CherOR7对Z8-14:OAc有反应，然而，未鉴定出对Z5-14:OAc的受体。除了Z8-14:OAc外，CherOR7还对Z7-14:OAc有反应，表明该受体可能受到的限制较宽松。在CoblOR7和CherOR7之间的29个氨基酸差异中，显著更多的差异位于第三和第六跨膜结构域区域。总体而言，这些发现与研究结果一致，即揭示了两种物种中都存在对Z8-14:OAc和Z5-14:OAc都有反应的神经元，但对于赫氏卷叶蛾雄性来说，目前不需要检测Z8-14:OAc的能力。
