Cattaneo Alberto Maria, Kwadha Charles A, Pullmann-Lindsley Heidi, Erdei Anna L, Pitts R Jason, Walker William B
Department of Plant Protection Biology, Chemical Ecology. Lomma - Campus Alnarp, Swedish University of Agricultural Sciences, 234 56, Alnarp, Sweden.
Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, 27695-7616, USA.
J Chem Ecol. 2025 Feb 25;51(2):28. doi: 10.1007/s10886-025-01579-1.
With the advent of semiochemical-based control strategies used to mitigate damage of agricultural pest moths, many studies have focused on the function of male-specific putative pheromone receptors (PRs). In this investigation, we instead isolated, heterologously expressed, and functionally characterized a female-biased candidate PR, CpomOR22, from the codling moth, Cydia pomonella. Using transgenic Drosophila melanogaster for single sensillum recording (SSR) and gas-chromatographic SSR, we tested both synthetic ligands and various apple headspace extracts, identifying saturated and unsaturated aldehydes (nonanal, decanal, undecanal, dodecanal; (Z)-4-undecenal and (Z)-6-undecenal) among the most active ligands. Parallel experiments expressing CpomOR22 in Xenopus oocytes confirmed the binding of nonanal, decanal and undecanal and revealed lactones (γ-undecalactone and δ-dodecalactone) and several carboxylic acids as additional active compounds. The renowned ecological importance of aldehydes for the codling moth and the potential for newly identified ligands, such as lactones, may inform innovative control strategies based on novel semiochemicals to interfere with the female-specific chemosensory systems of this insect.
随着用于减轻农业害虫蛾类危害的基于信息素的控制策略的出现，许多研究都集中在雄性特异性假定信息素受体（PRs）的功能上。在本研究中，我们转而从苹果蠹蛾（Cydia pomonella）中分离、异源表达并对一种雌性偏向的候选PR——CpomOR22进行了功能表征。我们使用转基因黑腹果蝇进行单感器记录（SSR）和气-相色谱SSR，测试了合成配体和各种苹果顶空提取物，确定了最具活性的配体中的饱和醛和不饱和醛（壬醛、癸醛、十一醛、十二醛；（Z）-4-十一烯醛和（Z）-6-十一烯醛）。在非洲爪蟾卵母细胞中表达CpomOR22的平行实验证实了壬醛、癸醛和十一醛的结合，并揭示了内酯（γ-十一烷内酯和δ-十二烷内酯）和几种羧酸为其他活性化合物。醛类对苹果蠹蛾具有重要的生态意义，以及新鉴定的配体（如内酯）的潜力，可能为基于新型信息素的创新控制策略提供参考，以干扰这种昆虫的雌性特异性化学感应系统。
